Improved tet-responsive promoters with minimized background expression

The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are indispensable to warrant low expression levels with the system turned "off". On the other hand, they must support high level of gene...

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Hauptverfasser: Loew, Rainer (VerfasserIn) , Heinz, Niels (VerfasserIn) , Hampf, Mathias (VerfasserIn) , Bujard, Hermann (VerfasserIn) , Gossen, Manfred (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 24 November 2010
In: BMC biotechnology
Year: 2010, Jahrgang: 10, Heft: 1, Pages: 1-13
ISSN:1472-6750
DOI:10.1186/1472-6750-10-81
Online-Zugang:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1186/1472-6750-10-81
Resolving-System, lizenzpflichtig, Volltext: https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-10-81
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Verfasserangaben:Rainer Loew, Niels Heinz, Mathias Hampf, Hermann Bujard, Manfred Gossen
Beschreibung
Zusammenfassung:The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are indispensable to warrant low expression levels with the system turned "off". On the other hand, they must support high level of gene expression in the "on"-state.
Beschreibung:Gesehen am 29.03.2023
Beschreibung:Online Resource
ISSN:1472-6750
DOI:10.1186/1472-6750-10-81