Improved tet-responsive promoters with minimized background expression
The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are indispensable to warrant low expression levels with the system turned "off". On the other hand, they must support high level of gene...
Gespeichert in:
| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
24 November 2010
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| In: |
BMC biotechnology
Year: 2010, Jahrgang: 10, Heft: 1, Pages: 1-13 |
| ISSN: | 1472-6750 |
| DOI: | 10.1186/1472-6750-10-81 |
| Online-Zugang: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1186/1472-6750-10-81 Resolving-System, lizenzpflichtig, Volltext: https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-10-81 
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| Verfasserangaben: | Rainer Loew, Niels Heinz, Mathias Hampf, Hermann Bujard, Manfred Gossen |
MARC
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| 520 | |a The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are indispensable to warrant low expression levels with the system turned "off". On the other hand, they must support high level of gene expression in the "on"-state. | ||
| 650 | 4 | |a Background Expression | |
| 650 | 4 | |a Fluorescence Activate Cell Sorting Analysis | |
| 650 | 4 | |a Luciferase Activity | |
| 650 | 4 | |a Minimal Promoter | |
| 650 | 4 | |a Transient Transfection Assay | |
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