Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformation
Mechanical properties of the chromatin-bearing nucleus in normal and pathological cells are of general interest for epigenetics and medicine. Conventional techniques for quantitative measurements of material properties of cellular matter are based on application of controlled forces onto the cellula...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 November 2010
|
| In: |
Journal of microscopy
Year: 2010, Jahrgang: 240, Heft: 3, Pages: 216-226 |
| ISSN: | 1365-2818 |
| DOI: | 10.1111/j.1365-2818.2010.03394.x |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1365-2818.2010.03394.x Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2818.2010.03394.x 
|
| Verfasserangaben: | E. Gladilin, M. Schulz, C. Kappel & R. Eils |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1841066729 | ||
| 003 | DE-627 | ||
| 005 | 20230710144252.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230403s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/j.1365-2818.2010.03394.x |2 doi | |
| 035 | |a (DE-627)1841066729 | ||
| 035 | |a (DE-599)KXP1841066729 | ||
| 035 | |a (OCoLC)1389806922 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Gladilin, Evgeny |e VerfasserIn |0 (DE-588)1162502290 |0 (DE-627)1026599474 |0 (DE-576)348573685 |4 aut | |
| 245 | 1 | 0 | |a Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformation |c E. Gladilin, M. Schulz, C. Kappel & R. Eils |
| 264 | 1 | |c 15 November 2010 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.04.2023 | ||
| 520 | |a Mechanical properties of the chromatin-bearing nucleus in normal and pathological cells are of general interest for epigenetics and medicine. Conventional techniques for quantitative measurements of material properties of cellular matter are based on application of controlled forces onto the cellular or nuclear boundary and do not allow probing intracellular structures that are not directly accessible for physical contact inside the living cell. In this work, we present a novel approach for contactless determination of the nuclear compressibility (i.e. the Poisson's ratio ν) in living cells by means of image- and model-based analysis of drug-induced cell deformation. The Poisson's ratio of the HeLa cell nucleus is determined from time-series of 3D images as a parameter of constitutive model that minimizes the dissimilarity between the numerically predicted and experimentally observed images. | ||
| 650 | 4 | |a 3D image analysis | |
| 650 | 4 | |a cell nucleus | |
| 650 | 4 | |a compressibility | |
| 650 | 4 | |a confocal scanning laser microscopy | |
| 650 | 4 | |a contactless deformation induction | |
| 650 | 4 | |a finite element method | |
| 650 | 4 | |a parameter estimation | |
| 700 | 1 | |a Schulz, Martin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kappel, C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Eils, Roland |d 1965- |e VerfasserIn |0 (DE-588)1020648287 |0 (DE-627)691291705 |0 (DE-576)361718195 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of microscopy |d Oxford [u.a.] : Wiley-Blackwell, 1969 |g 240(2010), 3, Seite 216-226 |h Online-Ressource |w (DE-627)320460959 |w (DE-600)2007259-4 |w (DE-576)091142911 |x 1365-2818 |7 nnas |a Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformation |
| 773 | 1 | 8 | |g volume:240 |g year:2010 |g number:3 |g pages:216-226 |g extent:11 |a Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformation |
| 856 | 4 | 0 | |u https://doi.org/10.1111/j.1365-2818.2010.03394.x |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2818.2010.03394.x |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230403 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 1020648287 |a Eils, Roland |m 1020648287:Eils, Roland |d 160000 |d 160100 |e 160000PE1020648287 |e 160100PE1020648287 |k 0/160000/ |k 1/160000/160100/ |p 4 |y j | ||
| 998 | |g 1162502290 |a Gladilin, Evgeny |m 1162502290:Gladilin, Evgeny |p 1 |x j | ||
| 999 | |a KXP-PPN1841066729 |e 4301287876 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 03.04.2023"],"relHost":[{"title":[{"subtitle":"principal publication of the Royal Microscopical Society ; official journal of the International Society for Stereology","title":"Journal of microscopy","title_sort":"Journal of microscopy"}],"pubHistory":["89.1969 -"],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"corporate":[{"role":"isb","display":"Royal Microscopical Society"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 05.10.2020"],"part":{"year":"2010","issue":"3","text":"240(2010), 3, Seite 216-226","extent":"11","pages":"216-226","volume":"240"},"id":{"eki":["320460959"],"issn":["1365-2818"],"zdb":["2007259-4"],"doi":["10.1111/(ISSN)1365-2818"]},"origin":[{"publisherPlace":"Oxford [u.a.] ; Oxford ; Oxford [u.a.]","dateIssuedKey":"1969","publisher":"Wiley-Blackwell ; Blackwell Science ; Blackwell","dateIssuedDisp":"1969-"}],"disp":"Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformationJournal of microscopy","recId":"320460959"}],"title":[{"title":"Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformation","title_sort":"Contactless determination of nuclear compressibility using 3D image- and model-based analysis of drug-induced cellular deformation"}],"person":[{"display":"Gladilin, Evgeny","family":"Gladilin","role":"aut","given":"Evgeny"},{"display":"Schulz, Martin","role":"aut","given":"Martin","family":"Schulz"},{"given":"C.","role":"aut","family":"Kappel","display":"Kappel, C."},{"display":"Eils, Roland","family":"Eils","given":"Roland","role":"aut"}],"physDesc":[{"extent":"11 S."}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedDisp":"15 November 2010","dateIssuedKey":"2010"}],"id":{"doi":["10.1111/j.1365-2818.2010.03394.x"],"eki":["1841066729"]},"recId":"1841066729","name":{"displayForm":["E. Gladilin, M. Schulz, C. Kappel & R. Eils"]}} | ||
| SRT | |a GLADILINEVCONTACTLES1520 | ||