Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS
Phosphatidylethanol (PEth) is an abnormal phospholipid carrying two fatty acid chains. It is only formed in the presence of ethanol via the action of phospholipase D (PLD). Its use as a biomarker for alcohol consumption is currently under investigation. Previous methods for the analysis of PEth incl...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2 February 2010
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| In: |
Analytical and bioanalytical chemistry
Year: 2010, Jahrgang: 396, Heft: 7, Pages: 2415-2423 |
| ISSN: | 1618-2650 |
| DOI: | 10.1007/s00216-010-3458-5 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00216-010-3458-5
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| Verfasserangaben: | H. Gnann, C. Engelmann, G. Skopp, M. Winkler, V. Auwärter, S. Dresen, N. Ferreirós, F.M. Wurst, W. Weinmann |
| Zusammenfassung: | Phosphatidylethanol (PEth) is an abnormal phospholipid carrying two fatty acid chains. It is only formed in the presence of ethanol via the action of phospholipase D (PLD). Its use as a biomarker for alcohol consumption is currently under investigation. Previous methods for the analysis of PEth included high-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD), which is unspecific for the different homologues—improved methods are now based on time of flight mass spectrometry (TOF-MS) and tandem mass spectrometry (MS/MS). The intention of this work was to identify as many homologues of PEth as possible. A screening procedure using multiple-reaction monitoring (MRM) for the identified homologues has subsequently been established. For our investigations, autopsy blood samples collected from heavy drinkers were used. Phosphatidylpropanol 16:0/18:1 (internal standard) was added to the blood samples prior to liquid-liquid extraction using borate buffer (pH 9), 2-propanol and n-hexane. After evaporation, the samples were redissolved in the mobile phase and injected into the LC-MS/MS system. Compounds were separated on a Luna Phenyl Hexyl column (50 mm × 2 mm, 3 µm) by gradient elution, using 2 mM ammonium acetate and methanol/acetone (95/5; v/v). A total of 48 homologues of PEth could be identified by using precursor ion and enhanced product ion scans (EPI). |
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| Beschreibung: | Gesehen am 05.04.2023 |
| Beschreibung: | Online Resource |
| ISSN: | 1618-2650 |
| DOI: | 10.1007/s00216-010-3458-5 |