A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila
Transcription factors of the RUNX and GATA families play key roles in the control of cell fate choice and differentiation, notably in the hematopoietic system. During Drosophila hematopoiesis, the RUNX factor Lozenge and the GATA factor Serpent cooperate to induce crystal cell differentiation. We us...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
Juni 2010,
|
| In: |
Molecular and cellular biology
Year: 2010, Jahrgang: 30, Heft: 11, Pages: 2837-2848 |
| ISSN: | 0270-7306 |
| DOI: | 10.1128/MCB.01625-09 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/MCB.01625-09 Verlag, lizenzpflichtig, Volltext: https://journals.asm.org/doi/10.1128/MCB.01625-09 
|
| Verfasserangaben: | Vanessa Gobert, Dani Osman, Stéphanie Bras, Benoit Augé, Muriel Boube, Henri-Marc Bourbon, Thomas Horn, Michael Boutros, Marc Haenlin, and Lucas Waltzer |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1841615803 | ||
| 003 | DE-627 | ||
| 005 | 20230710144213.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230405s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1128/MCB.01625-09 |2 doi | |
| 035 | |a (DE-627)1841615803 | ||
| 035 | |a (DE-599)KXP1841615803 | ||
| 035 | |a (OCoLC)1389806908 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Gobert, Vanessa |e VerfasserIn |4 aut | |
| 245 | 1 | 2 | |a A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila |c Vanessa Gobert, Dani Osman, Stéphanie Bras, Benoit Augé, Muriel Boube, Henri-Marc Bourbon, Thomas Horn, Michael Boutros, Marc Haenlin, and Lucas Waltzer |
| 264 | 1 | |c Juni 2010, | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 05.04.2023 | ||
| 520 | |a Transcription factors of the RUNX and GATA families play key roles in the control of cell fate choice and differentiation, notably in the hematopoietic system. During Drosophila hematopoiesis, the RUNX factor Lozenge and the GATA factor Serpent cooperate to induce crystal cell differentiation. We used Serpent/Lozenge-activated transcription as a paradigm to identify modulators of GATA/RUNX activity by a genome-wide RNA interference screen in cultured Drosophila blood cells. Among the 129 factors identified, several belong to the Mediator complex. Mediator is organized in three modules plus a regulatory “CDK8 module,” composed of Med12, Med13, CycC, and Cdk8, which has long been thought to behave as a single functional entity. Interestingly, our data demonstrate that Med12 and Med13 but not CycC or Cdk8 are essential for Serpent/Lozenge-induced transactivation in cell culture. Furthermore, our in vivo analysis of crystal cell development show that, while the four CDK8 module subunits control the emergence and the proliferation of this lineage, only Med12 and Med13 regulate its differentiation. We thus propose that Med12/Med13 acts as a coactivator for Serpent/Lozenge during crystal cell differentiation independently of CycC/Cdk8. More generally, we suggest that the set of conserved factors identified herein may regulate GATA/RUNX activity in mammals. | ||
| 700 | 1 | |a Osman, Dani |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bras, Stéphanie |e VerfasserIn |4 aut | |
| 700 | 1 | |a Augé, Benoit |e VerfasserIn |4 aut | |
| 700 | 1 | |a Boube, Muriel |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bourbon, Henri-Marc |e VerfasserIn |4 aut | |
| 700 | 1 | |a Horn, Thomas |e VerfasserIn |0 (DE-588)101956556X |0 (DE-627)690949367 |0 (DE-576)357310810 |4 aut | |
| 700 | 1 | |a Boutros, Michael |e VerfasserIn |0 (DE-588)1023031132 |0 (DE-627)717350045 |0 (DE-576)366291831 |4 aut | |
| 700 | 1 | |a Haenlin, Marc |e VerfasserIn |4 aut | |
| 700 | 1 | |a Waltzer, Lucas |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular and cellular biology |d Washington, DC : American Society for Microbiology, 1981 |g 30(2010), 11, Seite 2837-2848 |w (DE-627)130542199 |w (DE-600)779397-2 |w (DE-576)016104137 |x 0270-7306 |7 nnas |a A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila |
| 773 | 1 | 8 | |g volume:30 |g year:2010 |g number:11 |g pages:2837-2848 |g extent:12 |a A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila |
| 856 | 4 | 0 | |u https://doi.org/10.1128/MCB.01625-09 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://journals.asm.org/doi/10.1128/MCB.01625-09 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230405 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 1023031132 |a Boutros, Michael |m 1023031132:Boutros, Michael |d 60000 |e 60000PB1023031132 |k 0/60000/ |p 8 | ||
| 998 | |g 101956556X |a Horn, Thomas |m 101956556X:Horn, Thomas |d 50000 |e 50000PH101956556X |k 0/50000/ |p 7 | ||
| 999 | |a KXP-PPN1841615803 |e 4302985461 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"recId":"1841615803","type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 05.04.2023"],"title":[{"title":"A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila","title_sort":"genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila"}],"person":[{"display":"Gobert, Vanessa","roleDisplay":"VerfasserIn","role":"aut","family":"Gobert","given":"Vanessa"},{"display":"Osman, Dani","roleDisplay":"VerfasserIn","role":"aut","family":"Osman","given":"Dani"},{"roleDisplay":"VerfasserIn","display":"Bras, Stéphanie","role":"aut","family":"Bras","given":"Stéphanie"},{"display":"Augé, Benoit","roleDisplay":"VerfasserIn","role":"aut","family":"Augé","given":"Benoit"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Boube, Muriel","given":"Muriel","family":"Boube"},{"roleDisplay":"VerfasserIn","display":"Bourbon, Henri-Marc","role":"aut","family":"Bourbon","given":"Henri-Marc"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Horn, Thomas","given":"Thomas","family":"Horn"},{"role":"aut","display":"Boutros, Michael","roleDisplay":"VerfasserIn","given":"Michael","family":"Boutros"},{"display":"Haenlin, Marc","roleDisplay":"VerfasserIn","role":"aut","family":"Haenlin","given":"Marc"},{"family":"Waltzer","given":"Lucas","roleDisplay":"VerfasserIn","display":"Waltzer, Lucas","role":"aut"}],"relHost":[{"name":{"displayForm":["American Society for Microbiology"]},"id":{"issn":["0270-7306"],"zdb":["779397-2"],"eki":["130542199"]},"origin":[{"publisherPlace":"Washington, DC","publisher":"American Society for Microbiology","dateIssuedKey":"1981","dateIssuedDisp":"1981-2015"}],"language":["eng"],"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"American Society for Microbiology"}],"recId":"130542199","type":{"bibl":"periodical"},"disp":"A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in DrosophilaMolecular and cellular biology","part":{"pages":"2837-2848","issue":"11","year":"2010","extent":"12","text":"30(2010), 11, Seite 2837-2848","volume":"30"},"titleAlt":[{"title":"MCB"}],"pubHistory":["1.1981 - 35.2015"],"title":[{"title_sort":"Molecular and cellular biology","subtitle":"MCB","title":"Molecular and cellular biology"}]}],"physDesc":[{"extent":"12 S."}],"id":{"eki":["1841615803"],"doi":["10.1128/MCB.01625-09"]},"origin":[{"dateIssuedKey":"2010","dateIssuedDisp":"Juni 2010,"}],"name":{"displayForm":["Vanessa Gobert, Dani Osman, Stéphanie Bras, Benoit Augé, Muriel Boube, Henri-Marc Bourbon, Thomas Horn, Michael Boutros, Marc Haenlin, and Lucas Waltzer"]}} | ||
| SRT | |a GOBERTVANEGENOMEWIDE2010 | ||