Design and evaluation of genome-wide libraries for RNA interference screens
RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
15 June 2010
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| In: |
Genome biology
Year: 2010, Volume: 11, Issue: 6, Pages: 1-12 |
| ISSN: | 1474-760X |
| DOI: | 10.1186/gb-2010-11-6-r61 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1186/gb-2010-11-6-r61
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| Author Notes: | Thomas Horn, Thomas Sandmann and Michael Boutros |
MARC
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| 520 | |a RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/. | ||
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