Diabetes-related defects in sarcoplasmic Ca2+ release are prevented by inactivation of Gα11 and Gαq in murine cardiomyocytes
Neurohumoral stimulation of Gq-coupled receptors has been proposed as a central mechanism in the pathogenesis of diabetic heart disease. The resulting contractile dysfunction is closely related to abnormal intracellular Ca2+ handling with functional defects of the sarcoplasmic reticulum (SR). The pr...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
07 April 2010
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| In: |
Molecular and cellular biochemistry
Year: 2010, Jahrgang: 341, Pages: 235-244 |
| ISSN: | 1573-4919 |
| DOI: | 10.1007/s11010-010-0454-1 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11010-010-0454-1
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| Verfasserangaben: | Dieter Paul Hoyer, Sabine Grönke, Konrad F. Frank, Klaus Addicks, Nina Wettschureck, Stefan Offermanns, Erland Erdmann, Hannes Reuter |
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| 100 | 1 | |a Hoyer, Dieter Paul |d 1983- |e VerfasserIn |0 (DE-588)140343342 |0 (DE-627)618243941 |0 (DE-576)316504459 |4 aut | |
| 245 | 1 | 0 | |a Diabetes-related defects in sarcoplasmic Ca2+ release are prevented by inactivation of Gα11 and Gαq in murine cardiomyocytes |c Dieter Paul Hoyer, Sabine Grönke, Konrad F. Frank, Klaus Addicks, Nina Wettschureck, Stefan Offermanns, Erland Erdmann, Hannes Reuter |
| 246 | 3 | 3 | |a Diabetes-related defects in sarcoplasmic Ca 2+ release are prevented by inactivation of Gα 11 and Gα q in murine cardiomyocytes |
| 246 | 3 | 3 | |a Diabetes-related defects in sarcoplasmic Ca2+ release are prevented by inactivation of G alpha 11 and G alpha q in murine cardiomyocytes |
| 264 | 1 | |c 07 April 2010 | |
| 300 | |b Illustrationen | ||
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| 500 | |a 2+ in Ca2+ im Titel ist hochgestellt, 11 und q in Gα11 and Gαq sind tiefgestellt | ||
| 500 | |a Gesehen am 21.04.2023 | ||
| 520 | |a Neurohumoral stimulation of Gq-coupled receptors has been proposed as a central mechanism in the pathogenesis of diabetic heart disease. The resulting contractile dysfunction is closely related to abnormal intracellular Ca2+ handling with functional defects of the sarcoplasmic reticulum (SR). The present study was therefore designed to determine the role of Gq-protein signaling via Gα11 and Gαq in diabetes for the induction of functional and structural changes in the Ca2+ release complex of the SR. An experimental type 1-diabetes was induced in wild type, Gα11 knockout, and Gα11/q-knockout mice by injection of streptozotocin. Cardiac morphology and function was assessed in vivo by echocardiography. SR Ca2+ leak was tested in vitro based on a 45Ca2+ assay and protein densities as well as gene expression of ryanodine receptor (RyR2), FKBP12.6, sorcin, and annexin A7 were analyzed by immunoblot and RT-PCR. In wild type animals 8 weeks of diabetes resulted in cardiac hypertrophy and SR Ca2+ leak was increased. In addition, diabetic wild type animals showed reduced protein levels of FKBP12.6 and annexin A7. In Gα11- and Gα11/q-knockout animals, however, SR Ca2+ release and cardiac phenotype remained unchanged upon induction of diabetes. Densities of the proteins that we presently analyzed were also unaltered in Gα11-knockout mice. Gα11/q-knockout animals even showed increased expression of sorcin and annexin A7. Thus, based on the present study we suggest a signaling pathway via the Gq-proteins, Gα11 and Gαq, that could link increased neurohumoral stimulation in diabetes with defective RyR2 channel function by regulating protein expression of FKBP12.6, annexin A7, and sorcin. | ||
| 650 | 4 | |a Annexin A7 | |
| 650 | 4 | |a Knockout mice | |
| 650 | 4 | |a Ryanodine receptor | |
| 650 | 4 | |a Sorcin | |
| 650 | 4 | |a Type 1-diabetes | |
| 700 | 1 | |a Grönke, Sabine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Frank, Konrad F. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Addicks, Klaus |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wettschureck, Nina |d 1971- |e VerfasserIn |0 (DE-588)120748630 |0 (DE-627)080865828 |0 (DE-576)182697207 |4 aut | |
| 700 | 1 | |a Offermanns, Stefan |d 1964- |e VerfasserIn |0 (DE-588)113037279 |0 (DE-627)584488734 |0 (DE-576)289750113 |4 aut | |
| 700 | 1 | |a Erdmann, Erland |e VerfasserIn |4 aut | |
| 700 | 1 | |a Reuter, Hannes |e VerfasserIn |4 aut | |
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