Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair
A recent study suggested that human Cdc14B phosphatase has a central function in the G2 DNA damage checkpoint. In this study, we show that chicken DT40, human HCT116, and human telomerase reverse transcription-immortalized retinal pigment epithelial cells deleted for the Cdc14A or Cdc14B gene are DN...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
May 17 2010
|
| In: |
The journal of cell biology
Year: 2010, Jahrgang: 189, Heft: 4, Pages: 631-639 |
| ISSN: | 1540-8140 |
| DOI: | 10.1083/jcb.200910057 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1083/jcb.200910057
|
| Verfasserangaben: | Annamaria Mocciaro, Eli Berdougo, Kang Zeng, Elizabeth Black, Paola Vagnarelli, William Earnshaw, David Gillespie, Prasad Jallepalli, and Elmar Schiebel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1843698412 | ||
| 003 | DE-627 | ||
| 005 | 20230710141916.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230427s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1083/jcb.200910057 |2 doi | |
| 035 | |a (DE-627)1843698412 | ||
| 035 | |a (DE-599)KXP1843698412 | ||
| 035 | |a (OCoLC)1389806362 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Mocciaro, Annamaria |e VerfasserIn |0 (DE-588)143025317 |0 (DE-627)641853025 |0 (DE-576)334654378 |4 aut | |
| 245 | 1 | 0 | |a Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair |c Annamaria Mocciaro, Eli Berdougo, Kang Zeng, Elizabeth Black, Paola Vagnarelli, William Earnshaw, David Gillespie, Prasad Jallepalli, and Elmar Schiebel |
| 264 | 1 | |c May 17 2010 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.04.2023 | ||
| 520 | |a A recent study suggested that human Cdc14B phosphatase has a central function in the G2 DNA damage checkpoint. In this study, we show that chicken DT40, human HCT116, and human telomerase reverse transcription-immortalized retinal pigment epithelial cells deleted for the Cdc14A or Cdc14B gene are DNA damage checkpoint proficient and arrest efficiently in G2 in response to irradiation. Cdc14A knockout (KO) or Cdc14B-KO cells also maintain normal levels of Chk1 and Chk2 activation after irradiation. Surprisingly, however, irradiation-induced γ-H2A.X foci and DNA double-strand breaks persist longer in Cdc14A-KO or Cdc14B-KO cells than controls, suggesting that Cdc14 phosphatases are required for efficient DNA repair. | ||
| 700 | 1 | |a Berdougo, Eli |e VerfasserIn |4 aut | |
| 700 | 1 | |a Zeng, Kang |e VerfasserIn |4 aut | |
| 700 | 1 | |a Black, Elizabeth |e VerfasserIn |4 aut | |
| 700 | 1 | |a Vagnarelli, Paola |e VerfasserIn |4 aut | |
| 700 | 1 | |a Earnshaw, William |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gillespie, David |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jallepalli, Prasad |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schiebel, Elmar |d 1960- |e VerfasserIn |0 (DE-588)1034622595 |0 (DE-627)746029462 |0 (DE-576)165868597 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of cell biology |d New York, NY : Rockefeller Univ. Press, 1962 |g 189(2010), 4, Seite 631-639 |h Online-Ressource |w (DE-627)242065244 |w (DE-600)1421310-2 |w (DE-576)065026527 |x 1540-8140 |7 nnas |a Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair |
| 773 | 1 | 8 | |g volume:189 |g year:2010 |g number:4 |g pages:631-639 |g extent:9 |a Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair |
| 856 | 4 | 0 | |u https://doi.org/10.1083/jcb.200910057 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230427 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 1034622595 |a Schiebel, Elmar |m 1034622595:Schiebel, Elmar |d 700000 |d 706000 |e 700000PS1034622595 |e 706000PS1034622595 |k 0/700000/ |k 1/700000/706000/ |p 9 |y j | ||
| 998 | |g 143025317 |a Mocciaro, Annamaria |m 143025317:Mocciaro, Annamaria |d 140000 |e 140000PM143025317 |k 0/140000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1843698412 |e 4315151149 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1083/jcb.200910057"],"eki":["1843698412"]},"origin":[{"dateIssuedDisp":"May 17 2010","dateIssuedKey":"2010"}],"language":["eng"],"note":["Gesehen am 27.04.2023"],"title":[{"title":"Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair","title_sort":"Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair"}],"person":[{"role":"aut","family":"Mocciaro","display":"Mocciaro, Annamaria","given":"Annamaria"},{"given":"Eli","display":"Berdougo, Eli","family":"Berdougo","role":"aut"},{"family":"Zeng","display":"Zeng, Kang","given":"Kang","role":"aut"},{"family":"Black","given":"Elizabeth","display":"Black, Elizabeth","role":"aut"},{"role":"aut","family":"Vagnarelli","display":"Vagnarelli, Paola","given":"Paola"},{"display":"Earnshaw, William","given":"William","family":"Earnshaw","role":"aut"},{"family":"Gillespie","display":"Gillespie, David","given":"David","role":"aut"},{"role":"aut","family":"Jallepalli","given":"Prasad","display":"Jallepalli, Prasad"},{"role":"aut","given":"Elmar","display":"Schiebel, Elmar","family":"Schiebel"}],"name":{"displayForm":["Annamaria Mocciaro, Eli Berdougo, Kang Zeng, Elizabeth Black, Paola Vagnarelli, William Earnshaw, David Gillespie, Prasad Jallepalli, and Elmar Schiebel"]},"recId":"1843698412","relHost":[{"title":[{"subtitle":"JCB","title_sort":"journal of cell biology","title":"The journal of cell biology"}],"id":{"eki":["242065244"],"issn":["1540-8140"],"zdb":["1421310-2"]},"physDesc":[{"extent":"Online-Ressource"}],"recId":"242065244","note":["Gesehen am 20.08.26"],"language":["eng"],"origin":[{"publisherPlace":"New York, NY","publisher":"Rockefeller Univ. Press","dateIssuedKey":"1962","dateIssuedDisp":"1962-"}],"disp":"Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repairThe journal of cell biology","titleAlt":[{"title":"JCB"},{"title":"Cell biology"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"pages":"631-639","volume":"189","extent":"9","issue":"4","year":"2010","text":"189(2010), 4, Seite 631-639"},"pubHistory":["12.1962 -"]}],"physDesc":[{"extent":"9 S."}],"type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a MOCCIAROANVERTEBRATE1720 | ||