A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides
Background: Although cytological screening for cervical precancers has led to a reduction of cervical cancer incidence worldwide it is a subjective and variable method with low single-test sensitivity. New biomarkers like p16 that specifically highlight abnormal cervical cells can improve cytology p...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2010 Feb 4
|
| In: |
Cellular oncology
Year: 2010, Jahrgang: 32, Heft: 1-2, Pages: 109-119 |
| ISSN: | 1875-8606 |
| DOI: | 10.3233/CLO-2009-0508 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3233/CLO-2009-0508 Verlag, lizenzpflichtig, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237613/ 
|
| Verfasserangaben: | Niels Grabe, Bernd Lahrmann, Thora Pommerencke, Magnus von Knebel Doeberitz, Miriam Reuschenbach and Nicolas Wentzensen |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1843786796 | ||
| 003 | DE-627 | ||
| 005 | 20230710141718.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230428s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3233/CLO-2009-0508 |2 doi | |
| 035 | |a (DE-627)1843786796 | ||
| 035 | |a (DE-599)KXP1843786796 | ||
| 035 | |a (OCoLC)1389806065 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Grabe, Niels |e VerfasserIn |0 (DE-588)123210615 |0 (DE-627)716920697 |0 (DE-576)364937602 |4 aut | |
| 245 | 1 | 2 | |a A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides |c Niels Grabe, Bernd Lahrmann, Thora Pommerencke, Magnus von Knebel Doeberitz, Miriam Reuschenbach and Nicolas Wentzensen |
| 264 | 1 | |c 2010 Feb 4 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 28.04.2023 | ||
| 520 | |a Background: Although cytological screening for cervical precancers has led to a reduction of cervical cancer incidence worldwide it is a subjective and variable method with low single-test sensitivity. New biomarkers like p16 that specifically highlight abnormal cervical cells can improve cytology performance. Virtual microscopy offers an ideal platform for assisted evaluation and archiving of biomarker-stained slides., - Methods: We first performed a quantitative analysis of p16-stained slides digitized with the Hamamatsu NDP slide scanner. From the results an automated algorithm was created to reliably detect cells, nuclei and p16-stained cells. The algorithm's performance was evaluated on two complete slides and tiles from 52 independent slides (11,628, 4094 and 25,619 cells/clusters, respectively)., - Results: We achieved excellent performance to discriminate unstained cells from nuclei and biomarker-stained cells. The automated algorithm showed a high overall and positive agreement (99.0-99.7% and 70.9-83.4%, respectively) with the gold standard and had a very high sensitivity (89.1-100.0%) and specificity (98.9-100.0%) to detect biomarker-stained cells., - Conclusions: We implemented a virtual microscopy system allowing highly efficient automated prescreening and archiving of biomarker-stained slides. Based on the initial results, we will evaluate the performance of our system in large epidemiologic studies against disease endpoints. | ||
| 700 | 1 | |a Lahrmann, Bernd |d 1982- |e VerfasserIn |0 (DE-588)1022879715 |0 (DE-627)717324710 |0 (DE-576)366112821 |4 aut | |
| 700 | 1 | |a Pommerencke, Thora |d 1978- |e VerfasserIn |0 (DE-588)1034926748 |0 (DE-627)746499140 |0 (DE-576)382547098 |4 aut | |
| 700 | 1 | |a Knebel Doeberitz, Magnus von |e VerfasserIn |0 (DE-588)1022165291 |0 (DE-627)71693289X |0 (DE-576)364934239 |4 aut | |
| 700 | 1 | |a Reuschenbach, Miriam |d 1980- |e VerfasserIn |0 (DE-588)13879734X |0 (DE-627)703109723 |0 (DE-576)309094054 |4 aut | |
| 700 | 1 | |a Wentzensen, Nicolas |d 1973- |e VerfasserIn |0 (DE-588)124171753 |0 (DE-627)085684341 |0 (DE-576)18514683X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cellular oncology |d Amsterdam [u.a.] : IOS Press, 2004 |g 32(2010), 1-2, Seite 109-119 |h Online-Ressource |w (DE-627)392964430 |w (DE-600)2158254-3 |w (DE-576)273889974 |x 1875-8606 |7 nnas |a A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides |
| 773 | 1 | 8 | |g volume:32 |g year:2010 |g number:1-2 |g pages:109-119 |g extent:11 |a A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides |
| 856 | 4 | 0 | |u https://doi.org/10.3233/CLO-2009-0508 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237613/ |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230428 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 124171753 |a Wentzensen, Nicolas |m 124171753:Wentzensen, Nicolas |p 6 |y j | ||
| 998 | |g 13879734X |a Reuschenbach, Miriam |m 13879734X:Reuschenbach, Miriam |d 910000 |d 912000 |e 910000PR13879734X |e 912000PR13879734X |k 0/910000/ |k 1/910000/912000/ |p 5 | ||
| 998 | |g 1022165291 |a Knebel Doeberitz, Magnus von |m 1022165291:Knebel Doeberitz, Magnus von |d 910000 |d 912000 |e 910000PK1022165291 |e 912000PK1022165291 |k 0/910000/ |k 1/910000/912000/ |p 4 | ||
| 998 | |g 1034926748 |a Pommerencke, Thora |m 1034926748:Pommerencke, Thora |d 50000 |e 50000PP1034926748 |k 0/50000/ |p 3 | ||
| 998 | |g 1022879715 |a Lahrmann, Bernd |m 1022879715:Lahrmann, Bernd |d 50000 |e 50000PL1022879715 |k 0/50000/ |p 2 | ||
| 998 | |g 123210615 |a Grabe, Niels |m 123210615:Grabe, Niels |d 910000 |d 910100 |e 910000PG123210615 |e 910100PG123210615 |k 0/910000/ |k 1/910000/910100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1843786796 |e 4315978191 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"given":"Niels","family":"Grabe","role":"aut","display":"Grabe, Niels"},{"given":"Bernd","role":"aut","family":"Lahrmann","display":"Lahrmann, Bernd"},{"given":"Thora","role":"aut","family":"Pommerencke","display":"Pommerencke, Thora"},{"role":"aut","family":"Knebel Doeberitz","display":"Knebel Doeberitz, Magnus von","given":"Magnus von"},{"role":"aut","family":"Reuschenbach","display":"Reuschenbach, Miriam","given":"Miriam"},{"display":"Wentzensen, Nicolas","family":"Wentzensen","role":"aut","given":"Nicolas"}],"note":["Gesehen am 28.04.2023"],"language":["eng"],"origin":[{"dateIssuedDisp":"2010 Feb 4","dateIssuedKey":"2010"}],"name":{"displayForm":["Niels Grabe, Bernd Lahrmann, Thora Pommerencke, Magnus von Knebel Doeberitz, Miriam Reuschenbach and Nicolas Wentzensen"]},"id":{"eki":["1843786796"],"doi":["10.3233/CLO-2009-0508"]},"recId":"1843786796","type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"11 S."}],"title":[{"title_sort":"virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides","title":"A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides"}],"relHost":[{"language":["eng"],"pubHistory":["26.2004 - 32.2010"],"note":["Gesehen am 02.04.2025"],"titleAlt":[{"title":"Analytical cellular pathology"}],"origin":[{"dateIssuedKey":"2004","publisherPlace":"Amsterdam [u.a.]","dateIssuedDisp":"2004-2010","publisher":"IOS Press"}],"disp":"A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slidesCellular oncology","type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"zdb":["2158254-3"],"eki":["392964430"],"issn":["1875-8606"]},"recId":"392964430","title":[{"title":"Cellular oncology","subtitle":"the official journal of the International Society for Cellular Oncology","title_sort":"Cellular oncology"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"11","year":"2010","pages":"109-119","issue":"1-2","text":"32(2010), 1-2, Seite 109-119","volume":"32"}}]} | ||
| SRT | |a GRABENIELSVIRTUALMIC2010 | ||