Hyperglycemia impairs proteasome function by methylglyoxal
The ubiquitin-proteasome system is the main degradation machinery for intracellularly altered proteins. Hyperglycemia has been shown to increase intracellular levels of the reactive dicarbonyl methylglyoxal (MGO) in cells damaged by diabetes, resulting in modification of proteins and alterations of...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1 March 2010
|
| In: |
Diabetes
Year: 2009, Jahrgang: 59, Heft: 3, Pages: 670-678 |
| ISSN: | 1939-327X |
| DOI: | 10.2337/db08-1565 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2337/db08-1565
|
| Verfasserangaben: | Markus A. Queisser, Dachun Yao, Sven Geisler, Hans-Peter Hammes, Günter Lochnit, Erwin D. Schleicher, Michael Brownlee, and Klaus T. Preissner |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1844867110 | ||
| 003 | DE-627 | ||
| 005 | 20230710135218.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230509s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.2337/db08-1565 |2 doi | |
| 035 | |a (DE-627)1844867110 | ||
| 035 | |a (DE-599)KXP1844867110 | ||
| 035 | |a (OCoLC)1389805846 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Queisser, Markus Alexander |d 1976- |e VerfasserIn |0 (DE-588)138466858 |0 (DE-627)602600464 |0 (DE-576)307697665 |4 aut | |
| 245 | 1 | 0 | |a Hyperglycemia impairs proteasome function by methylglyoxal |c Markus A. Queisser, Dachun Yao, Sven Geisler, Hans-Peter Hammes, Günter Lochnit, Erwin D. Schleicher, Michael Brownlee, and Klaus T. Preissner |
| 264 | 1 | |c 1 March 2010 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Vorab veröffentlicht unter http://diabetes.diabetesjournals.org am 15. Dezember 2009 | ||
| 500 | |a Gesehen am 09.05.2023 | ||
| 520 | |a The ubiquitin-proteasome system is the main degradation machinery for intracellularly altered proteins. Hyperglycemia has been shown to increase intracellular levels of the reactive dicarbonyl methylglyoxal (MGO) in cells damaged by diabetes, resulting in modification of proteins and alterations of their function. In this study, the influence of MGO-derived advanced glycation end product (AGE) formation on the activity of the proteasome was investigated in vitro and in vivo.MGO-derived AGE modification of proteasome subunits was analyzed by mass spectrometry, immunoprecipitation, and Western blots. Proteasome activity was analyzed using proteasome-specific fluorogenic substrates. Experimental models included bovine retinal endothelial cells, diabetic Ins2Akita mice, glyoxalase 1 (GLO1) knockdown mice, and streptozotocin (STZ)-injected diabetic mice.In vitro incubation with MGO caused adduct formation on several 20S proteasomal subunit proteins. In cultured endothelial cells, the expression level of the catalytic 20S proteasome subunit was not altered but proteasomal chymotrypsin-like activity was significantly reduced. In contrast, levels of regulatory 19S proteasomal proteins were decreased. In diabetic Ins2Akita, STZ diabetic, and nondiabetic and diabetic G101 knockdown mice, chymotrypsin-like activity was also reduced and MGO modification of the 20S-β2 subunit was increased.Hyperglycemia-induced formation of MGO covalently modifies the 20S proteasome, decreasing its activity in the diabetic kidney and reducing the polyubiquitin receptor 19S-S5a. The results indicate a new link between hyperglycemia and impairment of cell functions. | ||
| 700 | 1 | |a Yao, Dachun |e VerfasserIn |4 aut | |
| 700 | 1 | |a Geisler, Sven |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hammes, Hans-Peter |d 1955- |e VerfasserIn |0 (DE-588)102028353X |0 (DE-627)691169772 |0 (DE-576)360007252 |4 aut | |
| 700 | 1 | |a Lochnit, Günter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schleicher, Erwin D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Brownlee, Michael |e VerfasserIn |4 aut | |
| 700 | 1 | |a Preissner, Klaus T. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Diabetes |d Alexandria, Va : Assoc., 1952 |g 59(2009), 3 vom: März, Seite 670-678 |h Online-Ressource |w (DE-627)30671521X |w (DE-600)1501252-9 |w (DE-576)082437432 |x 1939-327X |7 nnas |a Hyperglycemia impairs proteasome function by methylglyoxal |
| 773 | 1 | 8 | |g volume:59 |g year:2009 |g number:3 |g month:03 |g pages:670-678 |g extent:9 |a Hyperglycemia impairs proteasome function by methylglyoxal |
| 856 | 4 | 0 | |u https://doi.org/10.2337/db08-1565 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230509 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 102028353X |a Hammes, Hans-Peter |m 102028353X:Hammes, Hans-Peter |d 60000 |d 61400 |d 60000 |e 60000PH102028353X |e 61400PH102028353X |e 60000PH102028353X |k 0/60000/ |k 1/60000/61400/ |k 0/60000/ |p 4 | ||
| 999 | |a KXP-PPN1844867110 |e 4319975561 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"person":[{"role":"aut","given":"Markus Alexander","family":"Queisser","display":"Queisser, Markus Alexander"},{"display":"Yao, Dachun","family":"Yao","role":"aut","given":"Dachun"},{"given":"Sven","role":"aut","display":"Geisler, Sven","family":"Geisler"},{"display":"Hammes, Hans-Peter","family":"Hammes","given":"Hans-Peter","role":"aut"},{"given":"Günter","role":"aut","display":"Lochnit, Günter","family":"Lochnit"},{"role":"aut","given":"Erwin D.","family":"Schleicher","display":"Schleicher, Erwin D."},{"given":"Michael","role":"aut","display":"Brownlee, Michael","family":"Brownlee"},{"role":"aut","given":"Klaus T.","display":"Preissner, Klaus T.","family":"Preissner"}],"id":{"doi":["10.2337/db08-1565"],"eki":["1844867110"]},"physDesc":[{"extent":"9 S."}],"name":{"displayForm":["Markus A. Queisser, Dachun Yao, Sven Geisler, Hans-Peter Hammes, Günter Lochnit, Erwin D. Schleicher, Michael Brownlee, and Klaus T. Preissner"]},"recId":"1844867110","relHost":[{"part":{"volume":"59","extent":"9","text":"59(2009), 3 vom: März, Seite 670-678","issue":"3","pages":"670-678","year":"2009"},"language":["eng"],"corporate":[{"role":"isb","display":"American Diabetes Association"}],"id":{"eki":["30671521X"],"zdb":["1501252-9"],"issn":["1939-327X"]},"physDesc":[{"extent":"Online-Ressource"}],"recId":"30671521X","disp":"Hyperglycemia impairs proteasome function by methylglyoxalDiabetes","type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title":"Diabetes","title_sort":"Diabetes","subtitle":"a journal of the American Diabetes Association"}],"note":["Gesehen am 21.01.2022"],"pubHistory":["1.1952 -"],"origin":[{"publisherPlace":"Alexandria, Va","dateIssuedDisp":"1952-","publisher":"Assoc.","dateIssuedKey":"1952"}]}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"Hyperglycemia impairs proteasome function by methylglyoxal","title_sort":"Hyperglycemia impairs proteasome function by methylglyoxal"}],"origin":[{"dateIssuedDisp":"1 March 2010","dateIssuedKey":"2010"}],"note":["Vorab veröffentlicht unter http://diabetes.diabetesjournals.org am 15. Dezember 2009","Gesehen am 09.05.2023"]} | ||
| SRT | |a QUEISSERMAHYPERGLYCE1201 | ||