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Cellular origin of platelet-derived microparticles in vivo

Introduction - Microparticles (MP), presumably of platelet origin, are the most abundant microparticles in blood. To which extent such MP may also directly originate from megakaryocytes, however, is unknown. During hematopoietic stem cell transplantation, patients undergo total body irradiation whic...

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Hauptverfasser: Rank, Andreas (VerfasserIn) , Nieuwland, Rienk (VerfasserIn) , Delker, Ruth (VerfasserIn) , Köhler, Anton (VerfasserIn) , Toth, Bettina (VerfasserIn) , Pihusch, Verena (VerfasserIn) , Wilkowski, Ralf (VerfasserIn) , Pihusch, Rudolf (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 8 August 2010
In: Thrombosis research
Year: 2010, Jahrgang: 126, Heft: 4, Pages: e255-e259
ISSN:1879-2472
DOI:10.1016/j.thromres.2010.07.012
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.thromres.2010.07.012
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0049384810004032
Volltext
Verfasserangaben:Andreas Rank, Rienk Nieuwland, Ruth Delker, Anton Köhler, Bettina Toth, Verena Pihusch, Ralf Wilkowski, Rudolf Pihusch
Beschreibung
Zusammenfassung:Introduction - Microparticles (MP), presumably of platelet origin, are the most abundant microparticles in blood. To which extent such MP may also directly originate from megakaryocytes, however, is unknown. During hematopoietic stem cell transplantation, patients undergo total body irradiation which leads to an irreversible destruction of hematopoiesis. - Material and Methods - We studied the levels of “platelet-derived” MP (PMP) in 13 patients before and after total body irradiation with 12Gy (4Gy for 3days, dose rate 4.5cGy/min). PMP were isolated and double-stained with annexin V and anti-CD61. In 6 patients, we additionally analyzed MP exposing P-selectin or CD63. - Results - PMP rapidly declined upon total body irradiation, which was 2.4-fold faster than platelet disappearance. In contrast, the kinetics of MP exposing P-selectin or CD63 was comparable to platelets. - Conclusions - Since CD61-positive MP disappear faster than platelets or MP exposing P-selectin or CD63, our data indicate that MP exposing P-selectin or CD63 are likely to originate from platelets, whereas at least a major fraction of CD61-exposing MP is likely to originate from megakaryocytes in vivo.
Beschreibung:Gesehen am 10.05.2023
Beschreibung:Online Resource
ISSN:1879-2472
DOI:10.1016/j.thromres.2010.07.012

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