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Rhizoma coptidis inhibits LPS-induced MCP-1/CCL2 production in murine macrophages via an AP-1 and NFk B-dependent pathway

Introduction. The Chinese extract Rhizoma coptidis is well known for its anti-inflammatory, antioxidative, antiviral, and antimicrobial activity. The exact mechanisms of action are not fully understood. Methods. We examined the effect of the extract and its main compound, berberine, on LPS-induced i...

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Hauptverfasser: Remppis, Bjoern-Andrew (VerfasserIn) , Bea, Florian (VerfasserIn) , Greten, Henry Johannes (VerfasserIn) , Buttler, Annette (VerfasserIn) , Wang, Hongjie (VerfasserIn) , Zhou, Qianxing (VerfasserIn) , Preusch, Michael R. (VerfasserIn) , Enk, Ronny (VerfasserIn) , Ehehalt, Robert (VerfasserIn) , Katus, Hugo (VerfasserIn) , Blessing, Erwin (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 21 Jun 2010
In: Mediators of inflammation
Year: 2010, Jahrgang: 2010, Pages: 1-9
ISSN:1466-1861
DOI:10.1155/2010/194896
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1155/2010/194896
Verlag, lizenzpflichtig, Volltext: https://www.hindawi.com/journals/mi/2010/194896/
Volltext
Verfasserangaben:Andrew Remppis, Florian Bea, Henry Johannes Greten, Annette Buttler, Hongjie Wang, Qianxing Zhou, Michael R. Preusch, Ronny Enk, Robert Ehehalt, Hugo Katus, and Erwin Blessing
Beschreibung
Zusammenfassung:Introduction. The Chinese extract Rhizoma coptidis is well known for its anti-inflammatory, antioxidative, antiviral, and antimicrobial activity. The exact mechanisms of action are not fully understood. Methods. We examined the effect of the extract and its main compound, berberine, on LPS-induced inflammatory activity in a murine macrophage cell line. RAW 264.7 cells were stimulated with LPS and incubated with either Rhizoma coptidis extract or berberine. Activation of AP-1 and NF B was analyzed in nuclear extracts, secretion of MCP-1/CCL2 was measured in supernatants. Results. Incubation with Rhizoma coptidis and berberine strongly inhibited LPS-induced monocyte chemoattractant protein (MCP)-1 production in RAW cells. Activation of the transcription factors AP-1 and NF B was inhibited by Rhizoma coptidis in a dose- and time-dependent fashion. Conclusions. Rhizoma coptidis extract inhibits LPS-induced MCP-1/CCL2 production in vitro via an AP-1 and NF B-dependent pathway. Anti-inflammatory action of the extract is mediated mainly by its alkaloid compound berberine.
Beschreibung:Gesehen am 12.05.2023
Beschreibung:Online Resource
ISSN:1466-1861
DOI:10.1155/2010/194896

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