Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia
Sufficient oxygenation is indispensable for cognitive performance in mammals. In order to assure adequate oxygenation and to prevent hypoxia in medicine or aviation, different approaches of oxygen delivery are realized. With regard to hyperoxia, it is well known that it increases the risk of tissue...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
5 January 2010
|
| In: |
Molecular and cellular biochemistry
Year: 2010, Jahrgang: 339, Heft: 1, Pages: 9-21 |
| ISSN: | 1573-4919 |
| DOI: | 10.1007/s11010-009-0365-1 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11010-009-0365-1
|
| Verfasserangaben: | Jochen Hinkelbein, Robert E. Feldmann, Armin Kalenka |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1845370104 | ||
| 003 | DE-627 | ||
| 005 | 20240327080251.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230515s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11010-009-0365-1 |2 doi | |
| 035 | |a (DE-627)1845370104 | ||
| 035 | |a (DE-599)KXP1845370104 | ||
| 035 | |a (OCoLC)1389802054 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Hinkelbein, Jochen |d 1974-2025 |e VerfasserIn |0 (DE-588)123907381 |0 (DE-627)085516538 |0 (DE-576)185269095 |4 aut | |
| 245 | 1 | 0 | |a Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia |c Jochen Hinkelbein, Robert E. Feldmann, Armin Kalenka |
| 264 | 1 | |c 5 January 2010 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 15.05.2023 | ||
| 520 | |a Sufficient oxygenation is indispensable for cognitive performance in mammals. In order to assure adequate oxygenation and to prevent hypoxia in medicine or aviation, different approaches of oxygen delivery are realized. With regard to hyperoxia, it is well known that it increases the risk of tissue toxicity and inflammation by generating radical oxygen species. However, this impact of hyperoxia on the expression of specific brain proteins has not been evaluated in detail yet. The present study analyzes time-dependent changes in protein expression in rat brain after a short-term exposure to normobaric hyperoxia. Thirty-six Wistar rats were randomly assigned to six different groups, three normobaric hyperoxia (NH) groups or three normobaric normoxia (NN) groups, each consisting of n = 6 animals. NH animals were exposed to 100% oxygen, NN rats to 21% oxygen, each group for 3 h. One group of NH and one group of NN were killed immediately after the 3 h, one group each after 3 days and one group each after 7 days. Rat brains were removed for analysis and whole brain detergent protein lysates were separated via two-dimensional gel electrophoresis followed by subsequent identification of protein expression alterations by peptide mass fingerprinting using mass spectrometry. Also, a functional network mapping and molecular pathway analysis were carried out. Statistical analysis was performed using analysis of variance (ANOVA) with Bonferroni correction using P < 0.01. Physiological parameters of the animals did not differ significantly between the two groups except for partial oxygen pressure (580 vs. 89 mmHg; P < 0.05). The expression of nine proteins was found to be significantly altered (five up-regulated: GOT1, CCT2, TCP1, G6PD, and ALB; four down-regulated: PEBP1, PRDX2, ENO1, and MDH1). IPA generated a network with eight focus proteins associated with pathways in “cell death, cancer, and signalling”. Although hyperoxia was normobaric and induced for only 3 h, significant changes in brain protein expression were detectable immediately after the 3 h, after 3 days, as well as after 7 days. This may indicate effects on brain protein expression take place in the rat brain following a relatively short period of hyperoxia. | ||
| 650 | 4 | |a Brain | |
| 650 | 4 | |a Normobaric hyperoxia | |
| 650 | 4 | |a Oxygen toxicity | |
| 650 | 4 | |a Protein expression | |
| 650 | 4 | |a Proteomics | |
| 700 | 1 | |a Feldmann, Robert Enrico |d 1970- |e VerfasserIn |0 (DE-588)129555991 |0 (DE-627)47271578X |0 (DE-576)297720554 |4 aut | |
| 700 | 1 | |a Kalenka, Armin |d 1969-2025 |e VerfasserIn |0 (DE-588)120095548 |0 (DE-627)080443192 |0 (DE-576)292038798 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular and cellular biochemistry |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1973 |g 339(2010), 1 vom: Jan., Seite 9-21 |h Online-Ressource |w (DE-627)32042975X |w (DE-600)2003615-2 |w (DE-576)104082216 |x 1573-4919 |7 nnas |a Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia |
| 773 | 1 | 8 | |g volume:339 |g year:2010 |g number:1 |g month:01 |g pages:9-21 |g extent:13 |a Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s11010-009-0365-1 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230515 | ||
| 993 | |a Article | ||
| 994 | |a 2010 | ||
| 998 | |g 120095548 |a Kalenka, Armin |m 120095548:Kalenka, Armin |d 60000 |d 61600 |e 60000PK120095548 |e 61600PK120095548 |k 0/60000/ |k 1/60000/61600/ |p 3 |y j | ||
| 998 | |g 129555991 |a Feldmann, Robert Enrico |m 129555991:Feldmann, Robert Enrico |d 50000 |d 53200 |e 50000PF129555991 |e 53200PF129555991 |k 0/50000/ |k 1/50000/53200/ |p 2 | ||
| 998 | |g 123907381 |a Hinkelbein, Jochen |m 123907381:Hinkelbein, Jochen |p 1 |x j | ||
| 999 | |a KXP-PPN1845370104 |e 4321913691 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia","title_sort":"Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxia"}],"person":[{"role":"aut","display":"Hinkelbein, Jochen","roleDisplay":"VerfasserIn","given":"Jochen","family":"Hinkelbein"},{"display":"Feldmann, Robert Enrico","roleDisplay":"VerfasserIn","role":"aut","family":"Feldmann","given":"Robert Enrico"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Kalenka, Armin","given":"Armin","family":"Kalenka"}],"recId":"1845370104","language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 15.05.2023"],"id":{"eki":["1845370104"],"doi":["10.1007/s11010-009-0365-1"]},"origin":[{"dateIssuedDisp":"5 January 2010","dateIssuedKey":"2010"}],"name":{"displayForm":["Jochen Hinkelbein, Robert E. Feldmann, Armin Kalenka"]},"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"Dordrecht [u.a.] ; Dordrecht [u.a.]","publisher":"Springer Science + Business Media B.V ; Kluwer","dateIssuedKey":"1973","dateIssuedDisp":"1973-"}],"id":{"zdb":["2003615-2"],"eki":["32042975X"],"issn":["1573-4919"]},"pubHistory":["1.1973 -"],"part":{"volume":"339","text":"339(2010), 1 vom: Jan., Seite 9-21","extent":"13","year":"2010","issue":"1","pages":"9-21"},"note":["Gesehen am 18.10.05"],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Time-dependent alterations of cerebral proteins following short-term normobaric hyperoxiaMolecular and cellular biochemistry","recId":"32042975X","language":["eng"],"title":[{"subtitle":"an international journal for chemical biology in health and disease","title":"Molecular and cellular biochemistry","title_sort":"Molecular and cellular biochemistry"}]}],"physDesc":[{"extent":"13 S."}]} | ||
| SRT | |a HINKELBEINTIMEDEPEND5201 | ||