Enhancement of femtosecond laser-induced nucleation of protein in a gel solution
We found that the use of a gel solution with agarose enhanced femtosecond laser-induced nucleation and produced hen egg white lysozyme crystals at three to five times lower supersaturation than those by the femtosecond laser or agarose alone. The fast fluorescence imaging of the protein in the gel s...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 28 2010
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| In: |
Applied physics letters
Year: 2010, Volume: 96, Issue: 4, Pages: 1-3 |
| ISSN: | 1077-3118 |
| DOI: | 10.1063/1.3294622 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1063/1.3294622
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| Author Notes: | Ryota Murai, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori |
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| 245 | 1 | 0 | |a Enhancement of femtosecond laser-induced nucleation of protein in a gel solution |c Ryota Murai, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori |
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| 520 | |a We found that the use of a gel solution with agarose enhanced femtosecond laser-induced nucleation and produced hen egg white lysozyme crystals at three to five times lower supersaturation than those by the femtosecond laser or agarose alone. The fast fluorescence imaging of the protein in the gel solution revealed that cavitation bubbles created high-concentration regions at the focal point, which could be the trigger for protein nucleation. The lower diffusions of protein molecules in agarose gel retained the high-concentration regions for a longer time, and facilitated the nucleation. | ||
| 700 | 1 | |a Yoshikawa, Hiroshi |e VerfasserIn |0 (DE-588)1132516811 |0 (DE-627)887837336 |0 (DE-576)488839009 |4 aut | |
| 700 | 1 | |a Takahashi, Yoshinori |e VerfasserIn |4 aut | |
| 700 | 1 | |a Maruyama, Mihoko |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sugiyama, Shigeru |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sazaki, Gen |e VerfasserIn |4 aut | |
| 700 | 1 | |a Adachi, Hiroaki |e VerfasserIn |4 aut | |
| 700 | 1 | |a Takano, Kazufumi |e VerfasserIn |4 aut | |
| 700 | 1 | |a Matsumura, Hiroyoshi |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Mori, Yusuke |e VerfasserIn |4 aut | |
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