Identification of an important immunological difference between virulent varicella-zoster virus and its avirulent vaccine: viral disruption of dendritic cell instruction
Virulent varicella-zoster virus (VZV) can spread in immunocompetent humans, resulting in symptoms mostly of the skin. In contrast, vaccine Oka (V-Oka), the attenuated VZV vaccine strain, only rarely causes clinical reactions. The mechanisms underlying these pathogenetic differences are unclear. In t...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2010 Jul 1
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| In: |
The journal of immunology
Year: 2010, Volume: 185, Issue: 1, Pages: 488-497 |
| ISSN: | 1550-6606 |
| Online Access: |
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| Author Notes: | Cindy Gutzeit, Martin J. Raftery, Matthias Peiser, Karsten B. Tischer, Martina Ulrich, Melanie Eberhardt, Eggert Stockfleth, Thomas Giese, Andreas Sauerbrei, Craig T. Morita, and Günther Schönrich |
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| 245 | 1 | 0 | |a Identification of an important immunological difference between virulent varicella-zoster virus and its avirulent vaccine |b viral disruption of dendritic cell instruction |c Cindy Gutzeit, Martin J. Raftery, Matthias Peiser, Karsten B. Tischer, Martina Ulrich, Melanie Eberhardt, Eggert Stockfleth, Thomas Giese, Andreas Sauerbrei, Craig T. Morita, and Günther Schönrich |
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| 520 | |a Virulent varicella-zoster virus (VZV) can spread in immunocompetent humans, resulting in symptoms mostly of the skin. In contrast, vaccine Oka (V-Oka), the attenuated VZV vaccine strain, only rarely causes clinical reactions. The mechanisms underlying these pathogenetic differences are unclear. In this study, we comparatively analyzed the ability of virulent VZV and V-Oka to modulate instruction of dendritic cells (DCs) by innate signals. DCs isolated from normal human skin were susceptible to infection with VZV and V-Oka. Moreover, inflammatory DCs, which play a crucial role in the stimulation of Th1 immune responses, accumulated in herpes zoster lesions. Infection of inflammatory DCs generated in vitro with virulent VZV or V-Oka resulted in upregulation of CD1c. Upon coculture with CD1c-restricted innate cells, DCs developed a mature phenotype whether infected with virulent VZV or V-Oka. Intriguingly, a striking difference was detected on the functional level. The release of IFN-gamma and IL-12, the signature cytokines of Th1 responses, was enhanced by V-Oka but blocked by virulent VZV. V-Oka and virulent VZV efficiently synergized with CD40L, eliminating the possibility that CD40 signaling was a target of VZV-associated immune evasion. Instead, virulent VZV selectively interfered with signaling through TLR2, which is known to sense VZV. Thus, virulent VZV subverts Th1-promoting instruction of human DCs by blocking TLR2-mediated innate signals that prime IL-12 production by DCs. Taken together, our results demonstrate a novel immune-evasion mechanism of virulent VZV that has been lost during the attenuation process leading to the VZV vaccine strain. | ||
| 650 | 4 | |a Cells, Cultured | |
| 650 | 4 | |a Chickenpox Vaccine | |
| 650 | 4 | |a Coculture Techniques | |
| 650 | 4 | |a Dendritic Cells | |
| 650 | 4 | |a Herpes Zoster | |
| 650 | 4 | |a Herpesvirus 3, Human | |
| 650 | 4 | |a Herpesvirus Vaccines | |
| 650 | 4 | |a Humans | |
| 650 | 4 | |a Immune Evasion | |
| 650 | 4 | |a Interleukin-12 | |
| 650 | 4 | |a Middle Aged | |
| 650 | 4 | |a Monocytes | |
| 650 | 4 | |a Signal Transduction | |
| 650 | 4 | |a Th1 Cells | |
| 650 | 4 | |a Vaccines, Attenuated | |
| 650 | 4 | |a Virulence | |
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| 700 | 1 | |a Sauerbrei, Andreas |e VerfasserIn |0 (DE-588)109783409 |0 (DE-627)592607100 |0 (DE-576)303538066 |4 aut | |
| 700 | 1 | |a Morita, Craig T. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schönrich, Günther |e VerfasserIn |0 (DE-588)1178691152 |0 (DE-627)1049543777 |0 (DE-576)517861178 |4 aut | |
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