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Content aware image restoration improves spatiotemporal resolution in luminescence imaging

Luminescent reporters are due to their intrinsically high signal-to-noise ratio a powerful labelling tool for microscopy and macroscopic in vivo imaging in biomedical research. However, luminescence signal detection requires longer exposure times than fluorescence imaging and is consequently less su...

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Bibliographic Details
Main Authors: Boothe, Tobias (Author) , Ivanković, Mario (Author) , Grohme, Markus A. (Author) , Markus, M. Andrea (Author) , Dullin, Christian (Author) , Xu, Xingbo (Author) , Rink, Jochen C. (Author)
Format: Article (Journal)
Language:English
Published: 13 May 2023
In: Communications biology
Year: 2023, Volume: 6, Pages: 1-7
ISSN:2399-3642
DOI:10.1038/s42003-023-04886-z
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s42003-023-04886-z
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s42003-023-04886-z
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Author Notes:Tobias Boothe, Mario Ivanković, Markus A. Grohme, M. Andrea Markus, Christian Dullin, Xingbo Xu & Jochen C. Rink
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Summary:Luminescent reporters are due to their intrinsically high signal-to-noise ratio a powerful labelling tool for microscopy and macroscopic in vivo imaging in biomedical research. However, luminescence signal detection requires longer exposure times than fluorescence imaging and is consequently less suited for applications requiring high temporal resolution or throughput. Here we demonstrate that content aware image restoration can drastically reduce the exposure time requirements in luminescence imaging, thus overcoming one of the major limitations of the technique.
Item Description:Gesehen am 20.06.2023
Physical Description:Online Resource
ISSN:2399-3642
DOI:10.1038/s42003-023-04886-z

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