Binding partner switching on microtubules and Aurora-B in the mitosis to cytokinesis transition
The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2010
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| In: |
Molecular & cellular proteomics
Year: 2010, Jahrgang: 9, Heft: 2, Pages: 336-350 |
| ISSN: | 1535-9484 |
| DOI: | 10.1074/mcp.M900308-MCP200 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1074/mcp.M900308-MCP200 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1535947620338032 
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| Verfasserangaben: | Nurhan Özlü, Flavio Monigatti, Bernhard Y. Renard, Christine M. Field, Hanno Steen, Timothy J. Mitchison, and Judith J. Steen |
MARC
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| 245 | 1 | 0 | |a Binding partner switching on microtubules and Aurora-B in the mitosis to cytokinesis transition |c Nurhan Özlü, Flavio Monigatti, Bernhard Y. Renard, Christine M. Field, Hanno Steen, Timothy J. Mitchison, and Judith J. Steen |
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| 500 | |a This work was supported, in whole or in part, by National Institutes of Health Grant GM 23928 (to T. J. M.). This work was also supported by a junior faculty development award from Children’s Hospital Boston and Harvard Medical School (to J. J. S.) | ||
| 520 | |a The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that bind selectively to microtubules in C phase and identified several novel binding partners including nucleolar and spindle-associated protein. C phase-selective microtubule binding of many of these proteins depended on activity of Aurora kinases as assayed by treatment with the drug VX680. Aurora-B binding partners switched dramatically between M phase to C phase, and we identified several novel C phase-selective Aurora-B binding partners including PRC1, KIF4, and anaphase-promoting complex/cyclosome. Our approach can be extended to other cellular compartments and cell states, and our data provide the first broad biochemical framework for understanding C phase. Concretely, we report a central role for Aurora-B in regulating the C phase cytoskeleton. | ||
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