Tumor-associated macrophages respond to chemotherapy by detrimental transcriptional reprogramming and suppressing stabilin-1 mediated clearance of EGF
IntroductionTumor resistance to chemotherapy and metastatic relapse account for more than 90% of cancer specific mortality. Tumor-associated macrophages (TAMs) can process chemotherapeutic agents and impair their action. Little is known about the direct effects of chemotherapy on TAMs.MethodsThe eff...
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
07 March 2023
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| In: |
Frontiers in immunology
Year: 2023, Volume: 14, Pages: 1-15 |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2023.1000497 |
| Online Access: | Resolving-System, kostenfrei, Volltext: https://doi.org/10.3389/fimmu.2023.1000497 Verlag, kostenfrei, Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2023.1000497 
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| Author Notes: | Irina Larionova, Artem Kiselev, Elena Kazakova, Tengfei Liu, Marina Patysheva, Pavel Iamshchikov, Quan Liu, Dieuwertje M. Mossel, Vladimir Riabov, Militsa Rakina, Alexey Sergushichev, Natalia Bezgodova, Sergei Vtorushin, Nikolai Litviakov, Evgeny Denisov, Philipp Koshkin, Denis Pyankov, Matvei Tsyganov, Marina Ibragimova, Nadezhda Cherdyntseva and Julia Kzhyshkowska |
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| 245 | 1 | 0 | |a Tumor-associated macrophages respond to chemotherapy by detrimental transcriptional reprogramming and suppressing stabilin-1 mediated clearance of EGF |c Irina Larionova, Artem Kiselev, Elena Kazakova, Tengfei Liu, Marina Patysheva, Pavel Iamshchikov, Quan Liu, Dieuwertje M. Mossel, Vladimir Riabov, Militsa Rakina, Alexey Sergushichev, Natalia Bezgodova, Sergei Vtorushin, Nikolai Litviakov, Evgeny Denisov, Philipp Koshkin, Denis Pyankov, Matvei Tsyganov, Marina Ibragimova, Nadezhda Cherdyntseva and Julia Kzhyshkowska |
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| 520 | |a IntroductionTumor resistance to chemotherapy and metastatic relapse account for more than 90% of cancer specific mortality. Tumor-associated macrophages (TAMs) can process chemotherapeutic agents and impair their action. Little is known about the direct effects of chemotherapy on TAMs.MethodsThe effect of chemotherapeutic platinum agent cisplatin was assessed in the model system of human ex vivo TAMs. Whole-transcriptome sequencing for paired TAMs stimulated and not stimulated by cisplatin was analysed by NGS. Endocytic uptake of EGF was quantified by flow cytometry. Confocal microscopy was used to visualize stabilin-1-mediated internalization and endocytic trafficking of EGF in CHO cells expressing ectopically recombinant stabilin-1 and in stabilin-1+ TAMs. In cohort of patients with breast cancer, the effect of platinum therapy on the transcriptome of TAMs was validated, and differential expression of regulators of endocytosis was identified.ResultsHere we show that chemotherapeutic agent cisplatin can initiate detrimental transcriptional and functional programs in TAMs, without significant impairment of their viability. We focused on the clearance function of TAMs that controls composition of tumor microenvironment. For the first time we demonstrated that TAMs’ scavenger receptor stabilin-1 is responsible for the clearance of epidermal growth factor (EGF), a potent stimulator of tumor growth. Cisplatin suppressed both overall and EGF-specific endocytosis in TAMs by bidirectional mode: suppression of positive regulators and stimulation of negative regulators of endocytosis, with strongest effect on synaptotagmin-11 (SYT11), confirmed in patients with breast cancer.ConclusionOur data demonstrate that synergistic action of cytostatic agents and innovative immunomodulators is required to overcome cancer therapy resistance. | ||
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| 700 | 1 | |a Patysheva, Marina |e VerfasserIn |4 aut | |
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