Multi-channel colocalization analysis and visualization of viral proteins in fluorescence microscopy images
Automatic analysis of colocalizing biological structures in multi-channel fluorescence microscopy images is an important task to quantify and understand biological processes at high spatial-temporal resolution. Here, we introduce a software suite for colocalization analysis of spot-like objects in m...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2023
|
| In: |
IEEE access
Year: 2023, Jahrgang: 11, Pages: 49772-49784 |
| ISSN: | 2169-3536 |
| DOI: | 10.1109/ACCESS.2023.3276232 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://dx.doi.org/10.1109/ACCESS.2023.3276232
|
| Verfasserangaben: | Christian Ritter, Roman Thielemann, Ji-Young Lee, Minh Tu Pham, Ralf Bartenschlager, and Karl Rohr |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1853235342 | ||
| 003 | DE-627 | ||
| 005 | 20240307072652.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230721s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1109/ACCESS.2023.3276232 |2 doi | |
| 035 | |a (DE-627)1853235342 | ||
| 035 | |a (DE-599)KXP1853235342 | ||
| 035 | |a (OCoLC)1425214649 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Ritter, Christian |d 1989- |e VerfasserIn |0 (DE-588)1191138550 |0 (DE-627)1669590356 |4 aut | |
| 245 | 1 | 0 | |a Multi-channel colocalization analysis and visualization of viral proteins in fluorescence microscopy images |c Christian Ritter, Roman Thielemann, Ji-Young Lee, Minh Tu Pham, Ralf Bartenschlager, and Karl Rohr |
| 264 | 1 | |c 2023 | |
| 300 | |b Illustrationen | ||
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Veröffentlichungsdatum: 15. Mai 2023 | ||
| 500 | |a Gesehen am 21.07.2023 | ||
| 520 | |a Automatic analysis of colocalizing biological structures in multi-channel fluorescence microscopy images is an important task to quantify and understand biological processes at high spatial-temporal resolution. Here, we introduce a software suite for colocalization analysis of spot-like objects in multi-channel fluorescence microscopy images. The software suite consists of ColocQuant and ColocJ, and is easy to use for biologists. ColocQuant is a Python-based software with graphical user interface to quantify colocalization of particles in two or three channels. Object-based colocalization is performed by an efficient multi-dimensional graph-based k -d-tree approach, which determines nearest neighbors involved in double or triple colocalization. ColocJ enables efficient and intuitive visualization of the color composition of colocalizations by a Maxwell color triangle and a color ribbon. Colocalization information can be visualized for an entire image or a selected region-of-interest. In addition, global statistics of the particle intensity, particle size, and the number of colocalizations over time are provided. The colocalization analysis results can be exported and used in other software. We illustrate the application of our software suite for multi-channel live cell fluorescence microscopy image sequences of viral proteins in hepatitis C virus infected cells. We performed two-channel and three-channel colocalization analysis. | ||
| 650 | 4 | |a Biomedical imaging | |
| 650 | 4 | |a colocalization analysis | |
| 650 | 4 | |a Color | |
| 650 | 4 | |a Fluorescence | |
| 650 | 4 | |a Image color analysis | |
| 650 | 4 | |a Microscopy | |
| 650 | 4 | |a microscopy images | |
| 650 | 4 | |a viral proteins | |
| 650 | 4 | |a Viruses (medical) | |
| 650 | 4 | |a Visualization | |
| 700 | 1 | |a Thielemann, Roman |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lee, Ji Young |e VerfasserIn |0 (DE-588)1058274570 |0 (DE-627)79668037X |0 (DE-576)414323610 |4 aut | |
| 700 | 1 | |a Pham, Minh Tu |d 1989- |e VerfasserIn |0 (DE-588)1252772262 |0 (DE-627)1794415564 |4 aut | |
| 700 | 1 | |a Bartenschlager, Ralf |d 1958- |e VerfasserIn |0 (DE-588)1058097989 |0 (DE-627)796390509 |0 (DE-576)168706067 |4 aut | |
| 700 | 1 | |a Rohr, Karl |e VerfasserIn |0 (DE-588)137474466 |0 (DE-627)695829440 |0 (DE-576)303788593 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a Institute of Electrical and Electronics Engineers |t IEEE access |d New York, NY : IEEE, 2013 |g 11(2023), Seite 49772-49784 |h Online-Ressource |w (DE-627)728440385 |w (DE-600)2687964-5 |w (DE-576)373180713 |x 2169-3536 |7 nnas |
| 773 | 1 | 8 | |g volume:11 |g year:2023 |g pages:49772-49784 |g extent:13 |a Multi-channel colocalization analysis and visualization of viral proteins in fluorescence microscopy images |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1109/ACCESS.2023.3276232 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230721 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 137474466 |a Rohr, Karl |m 137474466:Rohr, Karl |d 160000 |d 160100 |e 160000PR137474466 |e 160100PR137474466 |k 0/160000/ |k 1/160000/160100/ |p 6 |y j | ||
| 998 | |g 1058097989 |a Bartenschlager, Ralf |m 1058097989:Bartenschlager, Ralf |d 910000 |d 911700 |e 910000PB1058097989 |e 911700PB1058097989 |k 0/910000/ |k 1/910000/911700/ |p 5 | ||
| 998 | |g 1252772262 |a Pham, Minh Tu |m 1252772262:Pham, Minh Tu |d 910000 |d 911700 |e 910000PP1252772262 |e 911700PP1252772262 |k 0/910000/ |k 1/910000/911700/ |p 4 | ||
| 998 | |g 1058274570 |a Lee, Ji Young |m 1058274570:Lee, Ji Young |d 910000 |d 911700 |e 910000PL1058274570 |e 911700PL1058274570 |k 0/910000/ |k 1/910000/911700/ |p 3 | ||
| 998 | |g 1191138550 |a Ritter, Christian |m 1191138550:Ritter, Christian |p 1 |x j | ||
| 999 | |a KXP-PPN1853235342 |e 4356599403 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2023","dateIssuedDisp":"2023"}],"note":["Veröffentlichungsdatum: 15. Mai 2023","Gesehen am 21.07.2023"],"name":{"displayForm":["Christian Ritter, Roman Thielemann, Ji-Young Lee, Minh Tu Pham, Ralf Bartenschlager, and Karl Rohr"]},"recId":"1853235342","title":[{"title_sort":"Multi-channel colocalization analysis and visualization of viral proteins in fluorescence microscopy images","title":"Multi-channel colocalization analysis and visualization of viral proteins in fluorescence microscopy images"}],"relHost":[{"pubHistory":["1.2013 -"],"part":{"pages":"49772-49784","text":"11(2023), Seite 49772-49784","volume":"11","extent":"13","year":"2023"},"type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"eki":["728440385"],"zdb":["2687964-5"],"issn":["2169-3536"]},"origin":[{"publisher":"IEEE","dateIssuedDisp":"2013-","publisherPlace":"New York, NY","dateIssuedKey":"2013"}],"recId":"728440385","titleAlt":[{"title":"Access"}],"title":[{"title":"IEEE access","subtitle":"practical research, open solutions","title_sort":"IEEE access"}],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"corporate":[{"display":"Institute of Electrical and Electronics Engineers","role":"aut"}],"note":["Gesehen am 24.10.12"],"disp":"Institute of Electrical and Electronics EngineersIEEE access","name":{"displayForm":["Institute of Electrical and Electronics Engineers"]}}],"physDesc":[{"noteIll":"Illustrationen","extent":"13 S."}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"doi":["10.1109/ACCESS.2023.3276232"],"eki":["1853235342"]},"person":[{"family":"Ritter","given":"Christian","display":"Ritter, Christian","role":"aut"},{"family":"Thielemann","given":"Roman","display":"Thielemann, Roman","role":"aut"},{"given":"Ji Young","family":"Lee","role":"aut","display":"Lee, Ji Young"},{"family":"Pham","given":"Minh Tu","display":"Pham, Minh Tu","role":"aut"},{"given":"Ralf","family":"Bartenschlager","role":"aut","display":"Bartenschlager, Ralf"},{"display":"Rohr, Karl","role":"aut","family":"Rohr","given":"Karl"}],"language":["eng"]} | ||
| SRT | |a RITTERCHRIMULTICHANN2023 | ||