Histological comparative analysis of Bowman layer grafts procured using 3 different techniques
Purpose: - The objective of this study is to perform a histological analysis of Bowman layer (BL) grafts. - Methods: - BL grafts were procured from 13 human cadaver corneal tissues using 3 different donor preparation techniques. Subsequently, the grafts were fixed in 10% buffered formalin phosp...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 2023
|
| In: |
Cornea
Year: 2023, Jahrgang: 42, Heft: 7, Pages: 888-893 |
| ISSN: | 1536-4798 |
| DOI: | 10.1097/ICO.0000000000003260 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1097/ICO.0000000000003260 Verlag, lizenzpflichtig, Volltext: https://journals.lww.com/corneajrnl/Abstract/2023/07000/Histological_Comparative_Analysis_of_Bowman_Layer.11.aspx 
|
| Verfasserangaben: | Hyeck-Soo Son, Loren Moon, Jiangxia Wang, Charles G. Eberhart, Albert S. Jun, Divya Srikumaran, Uri S. Soiberman |
MARC
| LEADER | 00000caa a22000002c 4500 | ||
|---|---|---|---|
| 001 | 1853474398 | ||
| 003 | DE-627 | ||
| 005 | 20240328081210.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230724s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1097/ICO.0000000000003260 |2 doi | |
| 035 | |a (DE-627)1853474398 | ||
| 035 | |a (DE-599)KXP1853474398 | ||
| 035 | |a (OCoLC)1425214396 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Son, Hyeck Soo |d 1991- |e VerfasserIn |0 (DE-588)1136747931 |0 (DE-627)893534676 |0 (DE-576)49081686X |4 aut | |
| 245 | 1 | 0 | |a Histological comparative analysis of Bowman layer grafts procured using 3 different techniques |c Hyeck-Soo Son, Loren Moon, Jiangxia Wang, Charles G. Eberhart, Albert S. Jun, Divya Srikumaran, Uri S. Soiberman |
| 264 | 1 | |c July 2023 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 24.07.2023 | ||
| 520 | |a Purpose: - The objective of this study is to perform a histological analysis of Bowman layer (BL) grafts. - Methods: - BL grafts were procured from 13 human cadaver corneal tissues using 3 different donor preparation techniques. Subsequently, the grafts were fixed in 10% buffered formalin phosphate and embedded in paraffin. Hematoxylin and eosin sections of BL grafts were obtained and analyzed under a light microscope. BL and full graft thickness were measured using an image-processing software. - Results: - All 13 BL grafts contained residual anterior stromal tissue. BL stripping using Kelman-McPherson and Moorfield forceps (technique 3) achieved the thinnest graft thickness with a mean full graft thickness of 18.7 μm (95% confidence interval [CI], −9.8 to 47.2) at the thinnest point of the graft, whereas BL procurement using the Melles lamellar dissector (technique 2) led to the highest mean full graft thickness of 279.9 μm (95% CI, 251.4-308.5) even at the thinnest area of the graft. By contrast, BL dissection using a blunt dissector (technique 1) provided a mean full graft thickness of 70.2 μm (95% CI, 40.4-100.1) at the graft's thinnest point. Although peripheral graft tears occurred in 50%, 50%, and 100% of techniques 1, 2, and 3, respectively, intact 6.25-mm diameter BL grafts could be secured in 50%, 100%, and 80% of techniques 1, 2, and 3, respectively. - Conclusions: - None of the techniques used led to the procurement of pure BL grafts devoid of the anterior stroma. Peripheral scoring with a thin needle and tissue manipulation with Kelman-McPherson and Moorfield forceps led to the thinnest grafts in this study. | ||
| 700 | 1 | |a Moon, Loren |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wang, Jiangxia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Eberhart, Charles G. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Jun, Albert S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Srikumaran, Divya |e VerfasserIn |4 aut | |
| 700 | 1 | |a Soiberman, Uri S. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cornea |d Philadelphia, Pa. : Lippincott, Williams & Wilkins, 1982 |g 42(2023), 7 vom: Juli, Seite 888-893 |h Online-Ressource |w (DE-627)328817384 |w (DE-600)2045943-9 |w (DE-576)094504180 |x 1536-4798 |7 nnas |a Histological comparative analysis of Bowman layer grafts procured using 3 different techniques |
| 773 | 1 | 8 | |g volume:42 |g year:2023 |g number:7 |g month:07 |g pages:888-893 |g extent:6 |a Histological comparative analysis of Bowman layer grafts procured using 3 different techniques |
| 856 | 4 | 0 | |u https://doi.org/10.1097/ICO.0000000000003260 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://journals.lww.com/corneajrnl/Abstract/2023/07000/Histological_Comparative_Analysis_of_Bowman_Layer.11.aspx |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230724 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 1136747931 |a Son, Hyeck Soo |m 1136747931:Son, Hyeck Soo |d 910000 |d 910900 |e 910000PS1136747931 |e 910900PS1136747931 |k 0/910000/ |k 1/910000/910900/ |p 1 |x j | ||
| 999 | |a KXP-PPN1853474398 |e 4357161053 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedDisp":"July 2023","dateIssuedKey":"2023"}],"relHost":[{"origin":[{"dateIssuedKey":"1982","publisherPlace":"Philadelphia, Pa.","publisher":"Lippincott, Williams & Wilkins","dateIssuedDisp":"1982-"}],"id":{"eki":["328817384"],"issn":["1536-4798"],"zdb":["2045943-9"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"the journal of cornea and external disease","title":"Cornea","title_sort":"Cornea"}],"recId":"328817384","name":{"displayForm":["Castroviejo Cornea Society"]},"disp":"Histological comparative analysis of Bowman layer grafts procured using 3 different techniquesCornea","type":{"bibl":"periodical","media":"Online-Ressource"},"pubHistory":["1.1982 -"],"note":["Gesehen am 20.11.14"],"part":{"volume":"42","year":"2023","issue":"7","pages":"888-893","extent":"6","text":"42(2023), 7 vom: Juli, Seite 888-893"},"language":["eng"]}],"id":{"doi":["10.1097/ICO.0000000000003260"],"eki":["1853474398"]},"physDesc":[{"extent":"6 S."}],"title":[{"title":"Histological comparative analysis of Bowman layer grafts procured using 3 different techniques","title_sort":"Histological comparative analysis of Bowman layer grafts procured using 3 different techniques"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 24.07.2023"],"person":[{"family":"Son","role":"aut","given":"Hyeck Soo","display":"Son, Hyeck Soo"},{"given":"Loren","display":"Moon, Loren","family":"Moon","role":"aut"},{"family":"Wang","role":"aut","given":"Jiangxia","display":"Wang, Jiangxia"},{"family":"Eberhart","role":"aut","display":"Eberhart, Charles G.","given":"Charles G."},{"given":"Albert S.","display":"Jun, Albert S.","family":"Jun","role":"aut"},{"display":"Srikumaran, Divya","given":"Divya","role":"aut","family":"Srikumaran"},{"display":"Soiberman, Uri S.","given":"Uri S.","family":"Soiberman","role":"aut"}],"recId":"1853474398","language":["eng"],"name":{"displayForm":["Hyeck-Soo Son, Loren Moon, Jiangxia Wang, Charles G. Eberhart, Albert S. Jun, Divya Srikumaran, Uri S. Soiberman"]}} | ||
| SRT | |a SONHYECKSOHISTOLOGIC2023 | ||