Mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene
The mature embryos of fourteen elite winter wheat cultivars have been transformed by a biolistic approach. The gene coding for γ-glutamylcysteine synthetase (EC 6.3.2.2) was used as a transgene in order to obtain stable transformants resistant to drought stress. A binary vector, pBinarUTRECS, was us...
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| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2010
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| In: |
Archives of biological sciences
Year: 2010, Volume: 62, Issue: 3, Pages: 539-546 |
| ISSN: | 1821-4339 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doiserbia.nb.rs/Article.aspx?ID=0354-46641003539G
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| Author Notes: | Vladislava Galović, T. Rausch and Slobodanka Gršić-Rausch |
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| 520 | |a The mature embryos of fourteen elite winter wheat cultivars have been transformed by a biolistic approach. The gene coding for γ-glutamylcysteine synthetase (EC 6.3.2.2) was used as a transgene in order to obtain stable transformants resistant to drought stress. A binary vector, pBinarUTRECS, was used. The gene was under the control of the CaMV35S promoter region. GUS::GFP gene fusion was used as a reporter system and nptII served as a selectable marker gene. A high regeneration capacity of callus tissue under the selective pressure and successful GUS assay of transformed tissue were an indication of successful insertion of a transgene into mature embryo derived wheat tissue. . | ||
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