A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco
In plants, transgenes containing Potato spindle tuber viroid (PSTVd) cDNA sequences were efficient targets of PSTVd infection-mediated RNA-directed DNA methylation. Here, we demonstrate that in PSTVd-infected tobacco plants, a 134 bp PSTVd fragment (PSTVd-134) did not become densely methylated when...
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| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
03 April 2010
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| In: |
Plant molecular biology
Year: 2010, Volume: 73, Issue: 4/5, Pages: 439-447 |
| ISSN: | 1573-5028 |
| DOI: | 10.1007/s11103-010-9631-6 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11103-010-9631-6
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| Author Notes: | Athanasios Dalakouras, Mirko Moser, Gabi Krczal, Michael Wassenegger |
MARC
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| 245 | 1 | 2 | |a A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco |c Athanasios Dalakouras, Mirko Moser, Gabi Krczal, Michael Wassenegger |
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| 520 | |a In plants, transgenes containing Potato spindle tuber viroid (PSTVd) cDNA sequences were efficient targets of PSTVd infection-mediated RNA-directed DNA methylation. Here, we demonstrate that in PSTVd-infected tobacco plants, a 134 bp PSTVd fragment (PSTVd-134) did not become densely methylated when it was inserted into a chimeric Satellite tobacco mosaic virus (STMV) construct. Only about 4-5% of all cytosines (Cs) of the PSTVd-134 were methylated when flanked by satellite sequences. In the same plants, C methylation was approximately 92% when the PSTVd-134 was in a PSTVd full length sequence context and roughly 33% when flanked at its 3' end by a 19 bp PSTVd and at its 5' end by a short viroid-unrelated sequence. In addition, PSTVd small interfering RNAs (siRNAs) produced from the replicating viroid failed to target PSTVd-134-containing chimeric STMV RNA for degradation. Satellite RNAs appear to have adopted secondary structures that protect them against RNA interference (RNAi)-mediated degradation. Protection can be extended to short non-satellite sequences residing in satellite RNAs, rendering them poor targets for nuclear and cytoplasmic RNAi induced in trans. | ||
| 650 | 4 | |a Base Sequence | |
| 650 | 4 | |a Blotting, Northern | |
| 650 | 4 | |a Blotting, Southern | |
| 650 | 4 | |a DNA Methylation | |
| 650 | 4 | |a DNA, Recombinant | |
| 650 | 4 | |a Gene Expression Regulation, Plant | |
| 650 | 4 | |a Gene Silencing | |
| 650 | 4 | |a Molecular Sequence Data | |
| 650 | 4 | |a Plant Viruses | |
| 650 | 4 | |a RNA Stability | |
| 650 | 4 | |a Sequence Analysis, DNA | |
| 650 | 4 | |a Tobacco | |
| 650 | 4 | |a Tobacco mosaic satellite virus | |
| 650 | 4 | |a Transgenes | |
| 650 | 4 | |a Viroids | |
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