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Tamoxifen-inducible Cre-mediated recombination in adipocytes

To generate a mouse line which allows inducible, Cre/loxP-dependent recombination in adipocytes, we used RedE/RedT-mediated recombineering to insert the CreERT2-transgene, which encodes a fusion protein of Cre and a mutated tamoxifen-responsive estrogen receptor, into the start codon of the adipocyt...

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Hauptverfasser: Sassmann, Antonia (VerfasserIn) , Offermanns, Stefan (VerfasserIn) , Wettschureck, Nina (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 16 August 2010
In: Genesis
Year: 2010, Jahrgang: 48, Heft: 10, Pages: 618-625
ISSN:1526-968X
DOI:10.1002/dvg.20665
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/dvg.20665
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/dvg.20665
Volltext
Verfasserangaben:Antonia Sassmann, Stefan Offermanns, and Nina Wettschureck

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520 |a To generate a mouse line which allows inducible, Cre/loxP-dependent recombination in adipocytes, we used RedE/RedT-mediated recombineering to insert the CreERT2-transgene, which encodes a fusion protein of Cre and a mutated tamoxifen-responsive estrogen receptor, into the start codon of the adipocyte-specific Adipoq gene. Adipoq encodes adiponectin, an adipokine specifically expressed in differentiated adipocytes. Tamoxifen treatment induced almost complete recombination in white adipose tissue of the AdipoqCreERT2 mouse line (97%-99%), while no recombination was seen in vehicle-treated animals. Recombination in brown adipose tissue was about 15%, whereas other organs and tissues did not undergo recombination. In addition, mice expressing CreERT2 in adipocytes did not show any alterations of metabolic functions like glucose tolerance, lipolysis, or energy expenditure compared to control mice. Therefore the AdipoqCreERT2 mouse line will be a valuable tool for studying the consequences of a temporally controlled deletion of floxed genes in white adipose tissue. genesis 48:618-625, 2010. © 2010 Wiley-Liss, Inc. 
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