Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injury
Introduction - Endothelial dysfunction is a potential side effect of brain death (BD). Ischemia/reperfusion (IR) injury during heart transplantation may lead to further endothelial damage. Protective effects of alpha-1-antitrypsin (AAT), a human neutrophil serine protease inhibitor, have been demons...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 2023
|
| In: |
Journal of surgical research
Year: 2023, Volume: 283, Pages: 953-964 |
| ISSN: | 1095-8673 |
| DOI: | 10.1016/j.jss.2022.11.047 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jss.2022.11.047 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022480422007880 
|
| Author Notes: | Qingwei Ding, MD, Sivakkanan Loganathan, MD, Pengyu Zhou, MD, Alex Ali Sayour, MD, Paige Brlecic, MD, Tamás Radovits, MD, PhD, Roxane Domain, MSc, Brice Korkmaz, PhD, Matthias Karck, MD, Gábor Szabó, MD, PhD, and Sevil Korkmaz-Icöz, PhD |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1855121018 | ||
| 003 | DE-627 | ||
| 005 | 20250901132709.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230810s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jss.2022.11.047 |2 doi | |
| 035 | |a (DE-627)1855121018 | ||
| 035 | |a (DE-599)KXP1855121018 | ||
| 035 | |a (OCoLC)1425212909 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Ding, Qingwei |d 1988- |e VerfasserIn |0 (DE-588)1229695990 |0 (DE-627)1751841316 |4 aut | |
| 245 | 1 | 0 | |a Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injury |c Qingwei Ding, MD, Sivakkanan Loganathan, MD, Pengyu Zhou, MD, Alex Ali Sayour, MD, Paige Brlecic, MD, Tamás Radovits, MD, PhD, Roxane Domain, MSc, Brice Korkmaz, PhD, Matthias Karck, MD, Gábor Szabó, MD, PhD, and Sevil Korkmaz-Icöz, PhD |
| 264 | 1 | |c March 2023 | |
| 300 | |b Illustrationen, Diagramme | ||
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online verfügbar: 9. Dezember 2022 | ||
| 500 | |a Gesehen am 10.08.2023 | ||
| 520 | |a Introduction - Endothelial dysfunction is a potential side effect of brain death (BD). Ischemia/reperfusion (IR) injury during heart transplantation may lead to further endothelial damage. Protective effects of alpha-1-antitrypsin (AAT), a human neutrophil serine protease inhibitor, have been demonstrated against IR injury. We hypothesized that AAT protects brain-dead rats’ vascular grafts from IR injury. - Methods - Donor rats were subjected to BD by inflation of a subdural balloon. After 5.5 h, aortic rings were immediately mounted in organ baths (BD, n = 6 rats) or preserved in saline, supplemented either with vehicle (BD-IR, n = 8 rats) or AAT (BD-IR + AAT, n = 14 rats) for 24 h. During organ bath experiment, rings from both IR groups were exposed to hypochlorite to simulate warm reperfusion-associated endothelial injury. Endothelial function was measured ex vivo. Immunohistochemical staining for caspases was carried out and DNA-strand breaks were evaluated using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Data are presented as median (interquartile range). - Results - AAT improved IR-induced decreased maximum endothelium-dependent vasorelaxation to acetylcholine in the BD-IR + AAT aortas compared to the BD-IR group (BD: 83 (9-28) % versus BD-IR: 49 (39-60) % versus BD-IR + AAT: 64 (24-42) %, P < 0.05). Additionally, an increase in the rings’ sensitivity to acetylcholine was noted after AAT (pD2-value: BD-IR + AAT: 7.35 (7.06-7.89) versus BD-IR: 6.96 (6.65-7.21), P < 0.05). Caspase-3, -8, -9, and -12 immunoreactivity and the number of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive cells were significantly decreased by AAT. - Conclusions - AAT alleviates endothelial dysfunction, prevents increased caspase-3, -8, -9, and -12 levels, and decreases apoptotic DNA breakage due to BD and IR injury. This suggests that AAT treatment may be therapeutically beneficial to reduce IR-induced vascular damage. | ||
| 540 | |q DE-3 |a Urheberrechtsschutz 1.0 |2 rs |u http://rightsstatements.org/vocab/InC/1.0/ | ||
| 650 | 4 | |a Alpha-1-antitrypsin | |
| 650 | 4 | |a Brain death | |
| 650 | 4 | |a Endothelial function | |
| 650 | 4 | |a Heart transplantation | |
| 650 | 4 | |a Ischemia/reperfusion | |
| 700 | 1 | |a Loganathan, Sivakkanan |d 1984- |e VerfasserIn |0 (DE-588)1036717585 |0 (DE-627)75102550X |0 (DE-576)385350856 |4 aut | |
| 700 | 1 | |8 1\p |a Zhou, Pengyu |d 1989- |e VerfasserIn |0 (DE-588)1200904729 |0 (DE-627)1683963628 |4 aut | |
| 700 | 1 | |8 2\p |a Sayour, Alex Ali |d 1994- |e VerfasserIn |0 (DE-588)1186074191 |0 (DE-627)1665335416 |4 aut | |
| 700 | 1 | |a Brlecic, Paige |d 1990- |e VerfasserIn |0 (DE-588)1104398672 |0 (DE-627)861861345 |0 (DE-576)47097124X |4 aut | |
| 700 | 1 | |8 3\p |a Radovits, Tamás |e VerfasserIn |0 (DE-588)1081943203 |0 (DE-627)846898101 |0 (DE-576)454965869 |4 aut | |
| 700 | 1 | |a Domain, Roxane |e VerfasserIn |4 aut | |
| 700 | 1 | |a Korkmaz, Brice |e VerfasserIn |4 aut | |
| 700 | 1 | |a Karck, Matthias |d 1961- |e VerfasserIn |0 (DE-588)130833525 |0 (DE-627)505909340 |0 (DE-576)298366169 |4 aut | |
| 700 | 1 | |a Szabó, Gábor |d 1969- |e VerfasserIn |0 (DE-588)1022955152 |0 (DE-627)717338681 |0 (DE-576)366215485 |4 aut | |
| 700 | 1 | |a Korkmaz-İçöz, Sevil |e VerfasserIn |0 (DE-588)1062748697 |0 (DE-627)806830336 |0 (DE-576)420147845 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of surgical research |d Orlando, Fla. : Academic Press, 1961 |g 283(2023) vom: März, Seite 953-964 |h Online-Ressource |w (DE-627)267836821 |w (DE-600)1470806-1 |w (DE-576)104193948 |x 1095-8673 |7 nnas |a Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injury |
| 773 | 1 | 8 | |g volume:283 |g year:2023 |g month:03 |g pages:953-964 |g extent:12 |a Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injury |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.jss.2022.11.047 |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0022480422007880 |x Verlag |z lizenzpflichtig |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20250301 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 2\p |a cgwrk |d 20250301 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 3\p |a cgwrk |d 20250301 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20230810 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 1062748697 |a Korkmaz-İçöz, Sevil |m 1062748697:Korkmaz-İçöz, Sevil |d 910000 |d 910200 |e 910000PK1062748697 |e 910200PK1062748697 |k 0/910000/ |k 1/910000/910200/ |p 11 |y j | ||
| 998 | |g 1022955152 |a Szabó, Gábor |m 1022955152:Szabó, Gábor |d 910000 |d 910200 |d 50000 |e 910000PS1022955152 |e 910200PS1022955152 |e 50000PS1022955152 |k 0/910000/ |k 1/910000/910200/ |k 0/50000/ |p 10 | ||
| 998 | |g 130833525 |a Karck, Matthias |m 130833525:Karck, Matthias |d 910000 |d 910200 |e 910000PK130833525 |e 910200PK130833525 |k 0/910000/ |k 1/910000/910200/ |p 9 | ||
| 998 | |g 1104398672 |a Brlecic, Paige |m 1104398672:Brlecic, Paige |p 5 | ||
| 998 | |g 1036717585 |a Loganathan, Sivakkanan |m 1036717585:Loganathan, Sivakkanan |d 50000 |e 50000PL1036717585 |k 0/50000/ |p 2 | ||
| 998 | |g 1229695990 |a Ding, Qingwei |m 1229695990:Ding, Qingwei |d 50000 |e 50000PD1229695990 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1855121018 |e 4364663735 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1855121018","physDesc":[{"noteIll":"Illustrationen, Diagramme","extent":"12 S."}],"person":[{"given":"Qingwei","role":"aut","family":"Ding","display":"Ding, Qingwei"},{"role":"aut","given":"Sivakkanan","family":"Loganathan","display":"Loganathan, Sivakkanan"},{"given":"Pengyu","role":"aut","family":"Zhou","display":"Zhou, Pengyu"},{"role":"aut","given":"Alex Ali","family":"Sayour","display":"Sayour, Alex Ali"},{"family":"Brlecic","role":"aut","given":"Paige","display":"Brlecic, Paige"},{"given":"Tamás","role":"aut","family":"Radovits","display":"Radovits, Tamás"},{"display":"Domain, Roxane","role":"aut","given":"Roxane","family":"Domain"},{"family":"Korkmaz","given":"Brice","role":"aut","display":"Korkmaz, Brice"},{"family":"Karck","given":"Matthias","role":"aut","display":"Karck, Matthias"},{"display":"Szabó, Gábor","given":"Gábor","role":"aut","family":"Szabó"},{"role":"aut","given":"Sevil","family":"Korkmaz-İçöz","display":"Korkmaz-İçöz, Sevil"}],"relHost":[{"disp":"Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injuryJournal of surgical research","physDesc":[{"extent":"Online-Ressource"}],"recId":"267836821","type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"note":["Gesehen am 30.01.2020"],"title":[{"subtitle":"JSR ; clinical and laboratory investigation ; official publication of the Association for Academic Surgery ; official publication of the Association of Veterans Administration Surgeons","title":"Journal of surgical research","title_sort":"Journal of surgical research"}],"origin":[{"publisher":"Academic Press","dateIssuedKey":"1961","dateIssuedDisp":"1961-","publisherPlace":"Orlando, Fla."}],"id":{"issn":["1095-8673"],"eki":["267836821"],"zdb":["1470806-1"]},"part":{"text":"283(2023) vom: März, Seite 953-964","volume":"283","extent":"12","pages":"953-964","year":"2023"},"corporate":[{"display":"Association for Academic Surgery","role":"isb"},{"display":"Association of Veterans Administration Surgeons","role":"isb"}],"titleAlt":[{"title":"JSR"}],"pubHistory":["1.1961 -"]}],"origin":[{"dateIssuedKey":"2023","dateIssuedDisp":"March 2023"}],"title":[{"title_sort":"Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injury","title":"Alpha-1-antitrypsin protects vascular grafts of brain-dead rats against ischemia/reperfusion injury"}],"note":["Online verfügbar: 9. Dezember 2022","Gesehen am 10.08.2023"],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Qingwei Ding, MD, Sivakkanan Loganathan, MD, Pengyu Zhou, MD, Alex Ali Sayour, MD, Paige Brlecic, MD, Tamás Radovits, MD, PhD, Roxane Domain, MSc, Brice Korkmaz, PhD, Matthias Karck, MD, Gábor Szabó, MD, PhD, and Sevil Korkmaz-Icöz, PhD"]},"id":{"eki":["1855121018"],"doi":["10.1016/j.jss.2022.11.047"]}} | ||
| SRT | |a DINGQINGWEALPHA1ANTI2023 | ||