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Pyrophosphate modulates plant stress responses via SUMOylation

Pyrophosphate (PPi), a byproduct of macromolecule biosynthesis is maintained at low levels by soluble inorganic pyrophosphatases (sPPase) found in all eukaryotes. In plants, H+-pumping pyrophosphatases (H+-PPase) convert the substantial energy present in PPi into an electrochemical gradient. We show...

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Main Authors: Patir, Meliha Gorkem (Author) , Andrés González, Zaida (Author) , Krebs, Melanie (Author) , Fink, Fabian (Author) , Drzewicka, Katarzyna (Author) , Stankovic-Valentin, Nicolas (Author) , Segami, Shoji (Author) , Schuck, Sebastian (Author) , Büttner, Michael (Author) , Hell, Rüdiger (Author) , Maeshima, Masayoshi (Author) , Melchior, Frauke (Author) , Schumacher, Karin (Author)
Format: Article (Journal)
Language:English
Published: Feb. 20, 2019
In: eLife
Year: 2019, Volume: 8, Pages: 1-23
ISSN:2050-084X
DOI:10.7554/eLife.44213
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.7554/eLife.44213
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Author Notes:M Görkem Patir-Nebioglu, Zaida Andrés, Melanie Krebs, Fabian Fink, Katarzyna Drzewicka, Nicolas Stankovic-Valentin, Shoji Segami, Sebastian Schuck, Michael Büttner, Rüdiger Hell, Masayoshi Maeshima, Frauke Melchior, Karin Schumacher
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Summary:Pyrophosphate (PPi), a byproduct of macromolecule biosynthesis is maintained at low levels by soluble inorganic pyrophosphatases (sPPase) found in all eukaryotes. In plants, H+-pumping pyrophosphatases (H+-PPase) convert the substantial energy present in PPi into an electrochemical gradient. We show here, that both cold- and heat stress sensitivity of fugu5 mutants lacking the major H+-PPase isoform AVP1 is correlated with reduced SUMOylation. In addition, we show that increased PPi concentrations interfere with SUMOylation in yeast and we provide evidence that SUMO activating E1-enzymes are inhibited by micromolar concentrations of PPi in a non-competitive manner. Taken together, our results do not only provide a mechanistic explanation for the beneficial effects of AVP1 overexpression in plants but they also highlight PPi as an important integrator of metabolism and stress tolerance.
Item Description:Gesehen am 17.08.2023
Physical Description:Online Resource
ISSN:2050-084X
DOI:10.7554/eLife.44213

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