The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase
Transcriptional repression mediated through histone deacetylation is a critical component of eukaryotic gene regulation. Here we demonstrate that the class II histone deacetylase HDAC4 is covalently modified by the ubiquitin-related SUMO-1 modifier. A sumoylation-deficient point mutant (HDAC4-K559R)...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
3 June 2002
|
| In: |
The EMBO journal
Year: 2002, Jahrgang: 21, Heft: 11, Pages: 2682-2691 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/21.11.2682 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1093/emboj/21.11.2682 Verlag, kostenfrei, Volltext: https://www.embopress.org/doi/full/10.1093/emboj/21.11.2682 
|
| Verfasserangaben: | Olivier Kirsh, Jacob-S. Seeler, Andrea Pichler, Andreas Gast, Stefan Müller, Eric Miska, Marion Mathieu, Annick Harel-Bellan, Tony Kouzarides, Frauke Melchior and Anne Dejean |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 185879045X | ||
| 003 | DE-627 | ||
| 005 | 20250113233629.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230905s2002 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/emboj/21.11.2682 |2 doi | |
| 035 | |a (DE-627)185879045X | ||
| 035 | |a (DE-599)KXP185879045X | ||
| 035 | |a (OCoLC)1425878179 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Kirsh, Olivier |e VerfasserIn |0 (DE-588)1301017094 |0 (DE-627)1858791030 |4 aut | |
| 245 | 1 | 4 | |a The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase |c Olivier Kirsh, Jacob-S. Seeler, Andrea Pichler, Andreas Gast, Stefan Müller, Eric Miska, Marion Mathieu, Annick Harel-Bellan, Tony Kouzarides, Frauke Melchior and Anne Dejean |
| 264 | 1 | |c 3 June 2002 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 05.09.2023 | ||
| 520 | |a Transcriptional repression mediated through histone deacetylation is a critical component of eukaryotic gene regulation. Here we demonstrate that the class II histone deacetylase HDAC4 is covalently modified by the ubiquitin-related SUMO-1 modifier. A sumoylation-deficient point mutant (HDAC4-K559R) shows a slightly impaired ability to repress transcription as well as reduced histone deacetylase activity. The ability of HDAC4 to self-aggregate is a prerequisite for proper sumoylation in vivo. Calcium/calmodulin-dependent protein kinase (CaMK) signalling, which induces nuclear export, abrogates SUMO-1 modification of HDAC4. Moreover, the modification depends on the presence of an intact nuclear localization signal and is catalysed by the nuclear pore complex (NPC) RanBP2 protein, a factor newly identified as a SUMO E3 ligase. These findings suggest that sumoylation of HDAC4 takes place at the NPC and is coupled to its nuclear import. Finally, modification experiments indicate that the MEF2-interacting transcription repressor (MITR) as well as HDAC1 and -6 are similarly SUMO modified, indicating that sumoylation may be an important regulatory mechanism for the control of transcriptional repression mediated by both class I and II HDACs. | ||
| 650 | 4 | |a E3 ligase | |
| 650 | 4 | |a HDACs | |
| 650 | 4 | |a nuclear pore complex | |
| 650 | 4 | |a SUMO | |
| 650 | 4 | |a ubiquitin-like modification | |
| 700 | 1 | |a Seeler, Jacob-S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pichler, Andrea |d 1966- |e VerfasserIn |0 (DE-588)1107797195 |0 (DE-627)863624960 |0 (DE-576)475134044 |4 aut | |
| 700 | 1 | |a Gast, Andreas |e VerfasserIn |0 (DE-588)1226448496 |0 (DE-627)1747463841 |4 aut | |
| 700 | 1 | |a Müller, Stefan |e VerfasserIn |4 aut | |
| 700 | 1 | |8 1\p |a Miska, Eric |e VerfasserIn |0 (DE-588)1261097386 |0 (DE-627)1870435389 |4 aut | |
| 700 | 1 | |a Mathieu, Marion |e VerfasserIn |4 aut | |
| 700 | 1 | |a Harel-Bellan, Annick |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kouzarides, Tony |e VerfasserIn |4 aut | |
| 700 | 1 | |a Melchior, Frauke |d 1962- |e VerfasserIn |0 (DE-588)1044947144 |0 (DE-627)773153624 |0 (DE-576)370602919 |4 aut | |
| 700 | 1 | |a Dejean, Anne |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a European Molecular Biology Organization |t The EMBO journal |d [London] : Nature Publishing Group UK, 1982 |g 21(2002), 11, Seite 2682-2691 |h Online-Ressource |w (DE-627)266022529 |w (DE-600)1467419-1 |w (DE-576)075961377 |x 1460-2075 |7 nnas |
| 773 | 1 | 8 | |g volume:21 |g year:2002 |g number:11 |g pages:2682-2691 |g extent:10 |a The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase |
| 856 | 4 | 0 | |u https://doi.org/10.1093/emboj/21.11.2682 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.embopress.org/doi/full/10.1093/emboj/21.11.2682 |x Verlag |z kostenfrei |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20230905 | ||
| 993 | |a Article | ||
| 994 | |a 2002 | ||
| 998 | |g 1044947144 |a Melchior, Frauke |m 1044947144:Melchior, Frauke |p 10 | ||
| 998 | |g 1226448496 |a Gast, Andreas |m 1226448496:Gast, Andreas |d 140000 |e 140000PG1226448496 |k 0/140000/ |p 4 | ||
| 999 | |a KXP-PPN185879045X |e 4372794606 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase","title":"The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase"}],"person":[{"family":"Kirsh","given":"Olivier","display":"Kirsh, Olivier","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Seeler","given":"Jacob-S.","display":"Seeler, Jacob-S.","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Pichler, Andrea","roleDisplay":"VerfasserIn","given":"Andrea","family":"Pichler"},{"family":"Gast","given":"Andreas","roleDisplay":"VerfasserIn","display":"Gast, Andreas","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Müller, Stefan","given":"Stefan","family":"Müller"},{"display":"Miska, Eric","roleDisplay":"VerfasserIn","role":"aut","family":"Miska","given":"Eric"},{"display":"Mathieu, Marion","roleDisplay":"VerfasserIn","role":"aut","family":"Mathieu","given":"Marion"},{"family":"Harel-Bellan","given":"Annick","display":"Harel-Bellan, Annick","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Kouzarides","given":"Tony","roleDisplay":"VerfasserIn","display":"Kouzarides, Tony","role":"aut"},{"family":"Melchior","given":"Frauke","roleDisplay":"VerfasserIn","display":"Melchior, Frauke","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Dejean, Anne","given":"Anne","family":"Dejean"}],"note":["Gesehen am 05.09.2023"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"185879045X","language":["eng"],"origin":[{"dateIssuedKey":"2002","dateIssuedDisp":"3 June 2002"}],"id":{"doi":["10.1093/emboj/21.11.2682"],"eki":["185879045X"]},"name":{"displayForm":["Olivier Kirsh, Jacob-S. Seeler, Andrea Pichler, Andreas Gast, Stefan Müller, Eric Miska, Marion Mathieu, Annick Harel-Bellan, Tony Kouzarides, Frauke Melchior and Anne Dejean"]},"physDesc":[{"extent":"10 S."}],"relHost":[{"pubHistory":["1.1982 -"],"titleAlt":[{"title":"The EMBO journal online"}],"part":{"text":"21(2002), 11, Seite 2682-2691","volume":"21","extent":"10","year":"2002","issue":"11","pages":"2682-2691"},"note":["Gesehen am 18.02.2016","Fortsetzung der Druckausgabe"],"disp":"European Molecular Biology OrganizationThe EMBO journal","type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"266022529","corporate":[{"display":"European Molecular Biology Organization","roleDisplay":"VerfasserIn","role":"aut"}],"language":["eng"],"title":[{"title_sort":"EMBO journal","title":"The EMBO journal"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisher":"Nature Publishing Group UK ; EMBO Press ; Oxford Univ. Press ; Nature Publ. Group ; Wiley","dateIssuedKey":"1982","dateIssuedDisp":"1982-","publisherPlace":"[London] ; Heidelberg ; Oxford [u.a.] ; London [u.a.] ; Hoboken, NJ [u.a.]"}],"id":{"issn":["1460-2075"],"zdb":["1467419-1"],"eki":["266022529"],"doi":["10.1002/(ISSN)1460-2075"]},"name":{"displayForm":["publ. for the European Molecular Biology Organization"]}}]} | ||
| SRT | |a KIRSHOLIVISUMOE3LIGA3200 | ||