RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae*
Ran/TC4 is a ras-related GTP-binding protein predominantly located in the nucleus. Ran/TC4 is essential for nuclear transport and is involved in mitotic control. In Saccharomyces cerevisiae a gene highly homologous to Ran/TC4 has been identified and named GSP1. Like all ras-related GTP-binding prote...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
19 May 1995
|
| In: |
The journal of biological chemistry
Year: 1995, Jahrgang: 270, Heft: 20, Pages: 11860-11865 |
| ISSN: | 1083-351X |
| DOI: | 10.1074/jbc.270.20.11860 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1074/jbc.270.20.11860 Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0021925817479178 
|
| Verfasserangaben: | Jörg Becker, Frauke Melchior, Volker Gerke, F. Ralf Bischoff, Herwig Ponstingl, Alfred Wittinghofer |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1858904439 | ||
| 003 | DE-627 | ||
| 005 | 20240311161654.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230906s1995 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1074/jbc.270.20.11860 |2 doi | |
| 035 | |a (DE-627)1858904439 | ||
| 035 | |a (DE-599)KXP1858904439 | ||
| 035 | |a (OCoLC)1425896343 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Becker, Jörg |e VerfasserIn |0 (DE-588)1301175463 |0 (DE-627)1858904684 |4 aut | |
| 245 | 1 | 0 | |a RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae* |c Jörg Becker, Frauke Melchior, Volker Gerke, F. Ralf Bischoff, Herwig Ponstingl, Alfred Wittinghofer |
| 264 | 1 | |c 19 May 1995 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 06.09.2023 | ||
| 520 | |a Ran/TC4 is a ras-related GTP-binding protein predominantly located in the nucleus. Ran/TC4 is essential for nuclear transport and is involved in mitotic control. In Saccharomyces cerevisiae a gene highly homologous to Ran/TC4 has been identified and named GSP1. Like all ras-related GTP-binding proteins, Gsp1p undergoes cycles of GTP hydrolysis and GDP/GTP exchange. The switching between the two different nucleotide bound states regulates the function of these GTP-binding proteins. Here we identify the product of the yeast RNA1 gene as the GTPase-activating protein (GAP) of Gsp1p. RNA1 belongs to a group of genes which are conserved in a variety of different organisms. We have expressed and purified recombinant Gsp1p and Rna1p from Escherichia coli. The GTPase activity of Gsp1p is stimulated 107-fold by Rna1p. In addition, we find that the previously identified human RanGAP1 and rna1p from Schizosaccharomyces pombe are also able to induce GTPase activity of Gsp1p. The GTP hydrolysis of Ran is induced by RanGAP1 and rna1p but not by Rna1p. Implications for the suggested functions of Ran/TC4/Gsp1p in nuclear transport and mitotic control are discussed. | ||
| 700 | 1 | |a Melchior, Frauke |d 1962- |e VerfasserIn |0 (DE-588)1044947144 |0 (DE-627)773153624 |0 (DE-576)370602919 |4 aut | |
| 700 | 1 | |a Gerke, Volker |e VerfasserIn |0 (DE-588)1301538817 |0 (DE-627)1859068618 |4 aut | |
| 700 | 1 | |a Bischoff, F. Ralf |d 1960- |e VerfasserIn |0 (DE-588)1169951783 |0 (DE-627)1037025091 |0 (DE-576)51233479X |4 aut | |
| 700 | 1 | |a Ponstingl, Herwig |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wittinghofer, Alfred |d 1943- |e VerfasserIn |0 (DE-588)1019388412 |0 (DE-627)690878664 |0 (DE-576)357938380 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of biological chemistry |d [Amsterdam] : Elsevier B.V., 1905 |g 270(1995), 20, Seite 11860-11865 |h Online-Ressource |w (DE-627)269247025 |w (DE-600)1474604-9 |w (DE-576)077883837 |x 1083-351X |7 nnas |a RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae* |
| 773 | 1 | 8 | |g volume:270 |g year:1995 |g number:20 |g pages:11860-11865 |g extent:6 |a RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae* |
| 856 | 4 | 0 | |u https://doi.org/10.1074/jbc.270.20.11860 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0021925817479178 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230906 | ||
| 993 | |a Article | ||
| 994 | |a 1995 | ||
| 998 | |g 1169951783 |a Bischoff, F. Ralf |m 1169951783:Bischoff, F. Ralf |d 140000 |e 140000PB1169951783 |k 0/140000/ |p 4 | ||
| 998 | |g 1044947144 |a Melchior, Frauke |m 1044947144:Melchior, Frauke |p 2 | ||
| 999 | |a KXP-PPN1858904439 |e 4373191565 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"origin":[{"publisherPlace":"[Amsterdam] ; Baltimore, Md. [u.a.] ; Bethesda, Md.","publisher":"Elsevier B.V. ; American Soc. of Biological Chemists ; ASBMB Publications","dateIssuedKey":"2021","dateIssuedDisp":"2021-"}],"id":{"zdb":["1474604-9"],"eki":["269247025"],"issn":["1083-351X"]},"name":{"displayForm":["publ. by the American Society for Biochemistry and Molecular Biology"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"journal of biological chemistry","title":"The journal of biological chemistry","subtitle":"JBC"}],"pubHistory":["1.1905/06 -"],"titleAlt":[{"title":"JBC online"},{"title":"JBC"}],"part":{"text":"270(1995), 20, Seite 11860-11865","volume":"270","extent":"6","year":"1995","issue":"20","pages":"11860-11865"},"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae*The journal of biological chemistry","note":["Gesehen am 19.05.2021"],"recId":"269247025","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"American Society for Biochemistry and Molecular Biology","role":"isb"}]}],"physDesc":[{"extent":"6 S."}],"id":{"doi":["10.1074/jbc.270.20.11860"],"eki":["1858904439"]},"origin":[{"dateIssuedKey":"1995","dateIssuedDisp":"19 May 1995"}],"name":{"displayForm":["Jörg Becker, Frauke Melchior, Volker Gerke, F. Ralf Bischoff, Herwig Ponstingl, Alfred Wittinghofer"]},"language":["eng"],"recId":"1858904439","note":["Gesehen am 06.09.2023"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae*","title_sort":"RNA1 Encodes a GTPase-activating Protein Specific for Gsp1p, the Ran/TC4 Homologue of Saccharomyces cerevisiae*"}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Becker, Jörg","given":"Jörg","family":"Becker"},{"display":"Melchior, Frauke","roleDisplay":"VerfasserIn","role":"aut","family":"Melchior","given":"Frauke"},{"roleDisplay":"VerfasserIn","display":"Gerke, Volker","role":"aut","family":"Gerke","given":"Volker"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Bischoff, F. Ralf","given":"F. Ralf","family":"Bischoff"},{"roleDisplay":"VerfasserIn","display":"Ponstingl, Herwig","role":"aut","family":"Ponstingl","given":"Herwig"},{"family":"Wittinghofer","given":"Alfred","roleDisplay":"VerfasserIn","display":"Wittinghofer, Alfred","role":"aut"}]} | ||
| SRT | |a BECKERJOERRNA1ENCODE1919 | ||