Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme
Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into...
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| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 30, 1990
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| In: |
FEBS letters
Year: 1990, Jahrgang: 268, Heft: 1, Pages: 17-20 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/0014-5793(90)80961-H |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1016/0014-5793(90)80961-H Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2890%2980961-H 
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| Verfasserangaben: | Frauke Melchior and Helmut Kindl |
| Zusammenfassung: | Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233-2 and pDS12/RBSII-2, respectively. Expression of the cDNA in Escherichia coli yielded an enzyniatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis. |
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| Beschreibung: | Gesehen am 07.09.2023 |
| Beschreibung: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/0014-5793(90)80961-H |