Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme
Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 30, 1990
|
| In: |
FEBS letters
Year: 1990, Jahrgang: 268, Heft: 1, Pages: 17-20 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/0014-5793(90)80961-H |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1016/0014-5793(90)80961-H Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2890%2980961-H 
|
| Verfasserangaben: | Frauke Melchior and Helmut Kindl |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1859050891 | ||
| 003 | DE-627 | ||
| 005 | 20240311171011.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230907s1990 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/0014-5793(90)80961-H |2 doi | |
| 035 | |a (DE-627)1859050891 | ||
| 035 | |a (DE-599)KXP1859050891 | ||
| 035 | |a (OCoLC)1425898404 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Melchior, Frauke |d 1962- |e VerfasserIn |0 (DE-588)1044947144 |0 (DE-627)773153624 |0 (DE-576)370602919 |4 aut | |
| 245 | 1 | 0 | |a Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme |c Frauke Melchior and Helmut Kindl |
| 264 | 1 | |c July 30, 1990 | |
| 300 | |a 4 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 07.09.2023 | ||
| 520 | |a Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233-2 and pDS12/RBSII-2, respectively. Expression of the cDNA in Escherichia coli yielded an enzyniatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis. | ||
| 650 | 4 | |a Bacterial expression | |
| 650 | 4 | |a Chalcone synthase | |
| 650 | 4 | |a Stilbene synthase sequence | |
| 650 | 4 | |a Vitis | |
| 700 | 1 | |a Kindl, Helmut |d 1936- |e VerfasserIn |0 (DE-588)1179290054 |0 (DE-627)1066793646 |0 (DE-576)518178927 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a Vereinigung der Europäischen Biochemischen Gesellschaften |t FEBS letters |d Chichester : Wiley, 1968 |g 268(1990), 1, Seite 17-20 |h Online-Ressource |w (DE-627)253779669 |w (DE-600)1460391-3 |w (DE-576)07279383X |x 1873-3468 |7 nnas |
| 773 | 1 | 8 | |g volume:268 |g year:1990 |g number:1 |g pages:17-20 |g extent:4 |a Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0014-5793(90)80961-H |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2890%2980961-H |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20230907 | ||
| 993 | |a Article | ||
| 994 | |a 1990 | ||
| 998 | |g 1044947144 |a Melchior, Frauke |m 1044947144:Melchior, Frauke |p 1 |x j | ||
| 999 | |a KXP-PPN1859050891 |e 4373553998 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Frauke Melchior and Helmut Kindl"]},"id":{"eki":["1859050891"],"doi":["10.1016/0014-5793(90)80961-H"]},"origin":[{"dateIssuedKey":"1990","dateIssuedDisp":"July 30, 1990"}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["Federation of European Biochemical Societies. Ed. M. Avron [u.a.]"]},"origin":[{"publisherPlace":"Chichester ; Amsterdam ; Amsterdam [u.a.]","publisher":"Wiley ; North-Holland Publ. Co. ; Elsevier","dateIssuedKey":"1968","dateIssuedDisp":"1968-"}],"id":{"zdb":["1460391-3"],"eki":["253779669"],"doi":["10.1002/(ISSN)1873-3468"],"issn":["1873-3468"]},"disp":"Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 18.12.2019","Fortsetzung der Druck-Ausgabe"],"language":["eng"],"corporate":[{"display":"Vereinigung der Europäischen Biochemischen Gesellschaften","roleDisplay":"VerfasserIn","role":"aut"}],"recId":"253779669","pubHistory":["1.1968 -"],"part":{"issue":"1","pages":"17-20","year":"1990","extent":"4","volume":"268","text":"268(1990), 1, Seite 17-20"},"title":[{"title_sort":"FEBS letters","title":"FEBS letters","subtitle":"for the rapid publication of short reports in biochemistry, biophysics, and molecular biology"}]}],"physDesc":[{"extent":"4 S."}],"person":[{"given":"Frauke","family":"Melchior","role":"aut","display":"Melchior, Frauke","roleDisplay":"VerfasserIn"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Kindl, Helmut","given":"Helmut","family":"Kindl"}],"title":[{"title":"Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme","title_sort":"Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme"}],"recId":"1859050891","language":["eng"],"note":["Gesehen am 07.09.2023"],"type":{"media":"Online-Ressource","bibl":"article-journal"}} | ||
| SRT | |a MELCHIORFRGRAPEVINES3019 | ||