Buchumschlag

Nuclear protein import in a permeabilized cell assay

An in vitro nuclear import assay, using digitonin-permeabilized cells, grown on cover slips, and supplemented with exogenous cytosol and fluorescent-transport ligand, was developed in our laboratory several years ago (1). For a general introduction into this technique and references for alternative...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
1. Verfasser: Melchior, Frauke (VerfasserIn)
Dokumenttyp: Kapitel/Artikel
Sprache:Englisch
Veröffentlicht: 1998
In: Protein targeting protocols
Year: 1998, Pages: 265-274
DOI:10.1385/0-89603-487-9:265
Online-Zugang:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1385/0-89603-487-9:265
Verlag, lizenzpflichtig, Volltext: https://link.springer.com/protocol/10.1385/0-89603-487-9:265
Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1385/0-89603-487-9:265
Volltext
Verfasserangaben:Frauke Melchior

MARC

LEADER 00000caa a2200000 c 4500
001 1859066283
003 DE-627
005 20240311143545.0
007 cr uuu---uuuuu
008 230907s1998 xx |||||o 00| ||eng c
024 7 |a 10.1385/0-89603-487-9:265  |2 doi 
035 |a (DE-627)1859066283 
035 |a (DE-599)KXP1859066283 
035 |a (OCoLC)1425890027 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Melchior, Frauke  |d 1962-  |e VerfasserIn  |0 (DE-588)1044947144  |0 (DE-627)773153624  |0 (DE-576)370602919  |4 aut 
245 1 0 |a Nuclear protein import in a permeabilized cell assay  |c Frauke Melchior 
264 1 |c 1998 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 07.09.2023 
520 |a An in vitro nuclear import assay, using digitonin-permeabilized cells, grown on cover slips, and supplemented with exogenous cytosol and fluorescent-transport ligand, was developed in our laboratory several years ago (1). For a general introduction into this technique and references for alternative assays developed by other laboratories, see refs. 2 and 3. Although the original assay faithfully reproduces transport, quantitation by methods such as densitometry on photographic negatives or ACAS (anchored cell analysis and sorting) interactive laser cytometry is a rather time-consuming process. Another disadvantage of the original assay involving attached cells is that the number of cells per assay cannot easily be controlled. Both problems are circumvented by using a modification of the import assay recently developed by Paschal and Gerace (4), and described in detail below. Here, suspension cells are used as the source for permeable cells, and relative transport rates are determined rapidly by flow cytometry (an ELISA-based quantitative assay that allows determination of molar transport rates is described in ref.5). 
650 4 |a FITC Dextran 
650 4 |a Import Assay 
650 4 |a Transport Buffer 
650 4 |a Transport Ligand 
650 4 |a Transport Reaction 
773 0 8 |i Enthalten in  |t Protein targeting protocols  |d Totowa, NJ : Humana Press, 1998  |g (1998), Seite 265-274  |h Online-Ressource (digital)  |w (DE-627)1651986215  |w (DE-576)377985317  |z 9781592595723  |7 nnam  |a Nuclear protein import in a permeabilized cell assay 
773 1 8 |g year:1998  |g pages:265-274  |g extent:10  |a Nuclear protein import in a permeabilized cell assay 
856 4 0 |u https://doi.org/10.1385/0-89603-487-9:265  |x Resolving-System  |x Verlag  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://link.springer.com/protocol/10.1385/0-89603-487-9:265  |x Verlag  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://doi.org/10.1385/0-89603-487-9:265  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20230907 
993 |a BookComponentPart 
994 |a 1998 
998 |g 1044947144  |a Melchior, Frauke  |m 1044947144:Melchior, Frauke  |p 1  |x j  |y j 
999 |a KXP-PPN1859066283  |e 4373645795 
BIB |a Y 
JSO |a {"person":[{"given":"Frauke","family":"Melchior","role":"aut","display":"Melchior, Frauke","roleDisplay":"VerfasserIn"}],"title":[{"title_sort":"Nuclear protein import in a permeabilized cell assay","title":"Nuclear protein import in a permeabilized cell assay"}],"type":{"media":"Online-Ressource","bibl":"chapter"},"note":["Gesehen am 07.09.2023"],"recId":"1859066283","language":["eng"],"name":{"displayForm":["Frauke Melchior"]},"origin":[{"dateIssuedKey":"1998","dateIssuedDisp":"1998"}],"id":{"doi":["10.1385/0-89603-487-9:265"],"eki":["1859066283"]},"physDesc":[{"extent":"10 S."}],"relHost":[{"recId":"1651986215","language":["eng"],"disp":"Nuclear protein import in a permeabilized cell assayProtein targeting protocols","type":{"bibl":"edited-book","media":"Online-Ressource"},"part":{"extent":"10","text":"(1998), Seite 265-274","pages":"265-274","year":"1998"},"person":[{"role":"edt","display":"Clegg, Roger A.","roleDisplay":"Hrsg.","given":"Roger A.","family":"Clegg"}],"title":[{"title":"Protein targeting protocols","title_sort":"Protein targeting protocols"}],"physDesc":[{"extent":"Online-Ressource (digital)"}],"relMultPart":[{"title":[{"title_sort":"Methods in molecular biology","title":"Methods in molecular biology"}],"note":["Gesehen am 05.11.12"],"type":{"bibl":"serial","media":"Online-Ressource"},"disp":"Methods in Molecular Biology","language":["eng"],"recId":"599671882","pubHistory":["1.1984 -"],"part":{"number_sort":["88"],"number":["88"]},"origin":[{"dateIssuedKey":"1984","publisher":"Humana Press ; [Springer]","dateIssuedDisp":"1984-","publisherPlace":"Totowa, NJ ; [Heidelberg]"}],"id":{"issn":["1940-6029"],"zdb":["2493551-7"],"eki":["599671882"]},"physDesc":[{"extent":"Online-Ressource"}],"dispAlt":"Methods in molecular biology"}],"name":{"displayForm":["ed. by Roger A. Clegg"]},"id":{"isbn":["9781592595723"],"doi":["10.1385/0896034879"],"eki":["1651986215"]},"origin":[{"publisherPlace":"Totowa, NJ","dateIssuedDisp":"1998","dateIssuedKey":"1998","publisher":"Humana Press"}]}]} 
SRT |a MELCHIORFRNUCLEARPRO1998 

Ähnliche Einträge