Genetic instability and diminished differentiation capacity in long-term cultured mouse neurosphere cells
The potential use of neural stem cells in basic research, drug testing and for development of therapeutic strategies requires large scale in vitro amplification, increasing the probability of genetic instability and transformation. Little is known, however, about potential correlations between long-...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
13 January 2010
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| In: |
Mechanisms of ageing and development
Year: 2010, Volume: 131, Issue: 2, Pages: 124-132 |
| ISSN: | 1872-6216 |
| DOI: | 10.1016/j.mad.2010.01.001 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.mad.2010.01.001 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0047637410000199 
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| Author Notes: | Vladimir Vukicevic, Anna Jauch, Timo C. Dinger, Linda Gebauer, Veronika Hornich, Stefan R. Bornstein, Monika Ehrhart-Bornstein, Albrecht M. Müller |
| Summary: | The potential use of neural stem cells in basic research, drug testing and for development of therapeutic strategies requires large scale in vitro amplification, increasing the probability of genetic instability and transformation. Little is known, however, about potential correlations between long-term culture of neural stem and progenitor cells (NSPCs), changed differentiation and self-renewal capacities, and the occurrence of chromosomal instability. This study investigates the effect of extended culture time on self-renewal, differentiation capacity, cell cycle phase distribution, telomere length, telomerase activity and chromosomal stability on fetal brain-derived cells that form floating sphere colonies (neurospheres)... |
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| Item Description: | Gesehen am 19.09.2023 |
| Physical Description: | Online Resource |
| ISSN: | 1872-6216 |
| DOI: | 10.1016/j.mad.2010.01.001 |