A fluorometric assay to determine labile copper(II) ions in serum
Labile copper(II) ions (Cu2+) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu2+ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson’s disease (WD) or neurological disorders. S...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
07 August 2023
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| In: |
Scientific reports
Year: 2023, Jahrgang: 13, Pages: 1-10 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-023-39841-9 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41598-023-39841-9 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41598-023-39841-9 
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| Verfasserangaben: | Maria Maares, Alessia Haupt, Christoph Schüßler, Marcel Kulike-Koczula, Julian Hackler, Claudia Keil, Isabelle Mohr, Lutz Schomburg, Roderich D. Süssmuth, Hans Zischka, Uta Merle & Hajo Haase |
MARC
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| 520 | |a Labile copper(II) ions (Cu2+) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu2+ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson’s disease (WD) or neurological disorders. So far, no direct quantification method has been described to determine this small Cu2+ subset. This study introduces a fluorometric high throughput assay using the novel Cu2+ binding fluoresceine-peptide sensor FP4 (Kd of the Cu2+-FP4-complex 0.38 pM) to determine labile Cu2+ in human and rat serum. ... | ||
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