The pH-dependent lactose metabolism of Lactobacillus delbrueckii subsp. bulgaricus: an integrative view through a mechanistic computational model
The fermentation process of milk to yoghurt using Lactobacillus delbrueckii subsp. bulgaricus in co-culture with Streptococcus thermophilus is hallmarked by the breakdown of lactose to organic acids such as lactate. This leads to a substantial decrease in pH - both in the medium, as well as cytosoli...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
10 September 2023
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| In: |
Journal of biotechnology
Year: 2023, Volume: 374, Pages: 90-100 |
| ISSN: | 1873-4863 |
| DOI: | 10.1016/j.jbiotec.2023.08.001 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.jbiotec.2023.08.001 Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0168165623001414 
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| Author Notes: | Tamara Bendig, Andreas Ulmer, Laura Luzia, Susanne Müller, Sven Sahle, Frank T. Bergmann, Maren Lösch, Florian Erdemann, Ahmad A. Zeidan, Sebastian N. Mendoza, Bas Teusink, Ralf Takors, Ursula Kummer, Ana Sofia Figueiredo |
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| 245 | 1 | 4 | |a The pH-dependent lactose metabolism of Lactobacillus delbrueckii subsp. bulgaricus |b an integrative view through a mechanistic computational model |c Tamara Bendig, Andreas Ulmer, Laura Luzia, Susanne Müller, Sven Sahle, Frank T. Bergmann, Maren Lösch, Florian Erdemann, Ahmad A. Zeidan, Sebastian N. Mendoza, Bas Teusink, Ralf Takors, Ursula Kummer, Ana Sofia Figueiredo |
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| 520 | |a The fermentation process of milk to yoghurt using Lactobacillus delbrueckii subsp. bulgaricus in co-culture with Streptococcus thermophilus is hallmarked by the breakdown of lactose to organic acids such as lactate. This leads to a substantial decrease in pH - both in the medium, as well as cytosolic. The latter impairs metabolic activities due to the pH-dependence of enzymes, which compromises microbial growth. To quantitatively elucidate the impact of the acidification on metabolism of L. bulgaricus in an integrated way, we have developed a proton-dependent computational model of lactose metabolism and casein degradation based on experimental data. The model accounts for the influence of pH on enzyme activities as well as cellular growth and proliferation of the bacterial population. We used a machine learning approach to quantify the cell volume throughout fermentation. Simulation results show a decrease in metabolic flux with acidification of the cytosol. Additionally, the validated model predicts a similar metabolic behaviour within a wide range of non-limiting substrate concentrations. This computational model provides a deeper understanding of the intricate relationships between metabolic activity and acidification and paves the way for further optimization of yoghurt production under industrial settings. | ||
| 650 | 4 | |a Cytosolic pH | |
| 650 | 4 | |a Intracellular pH | |
| 650 | 4 | |a Kinetic model | |
| 650 | 4 | |a Lactic acid bacteria | |
| 650 | 4 | |a Lactose metabolism | |
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| 700 | 1 | |a Luzia, Laura |e VerfasserIn |4 aut | |
| 700 | 1 | |a Müller, Susanne |e VerfasserIn |4 aut | |
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