Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cells
In arteries and arterioles, a chronic increase in blood pressure raises wall tension. This continuous biomechanical strain causes a change in gene expression in vascular smooth muscle cells (VSMCs) that may lead to pathological changes. Here we have characterised the functional properties of lipoma-...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2023
|
| In: |
Cells
Year: 2023, Jahrgang: 12, Heft: 18, Pages: 1-21 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells12182315 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.3390/cells12182315 Verlag, kostenfrei, Volltext: https://www.mdpi.com/2073-4409/12/18/2315 
|
| Verfasserangaben: | Alexandra Sporkova, Taslima Nahar, Mingsi Cao, Subhajit Ghosh, Carla Sens-Albert, Prisca Amayi Patricia Friede, Anika Nagel, Jaafar Al-Hasani and Markus Hecker |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1867274558 | ||
| 003 | DE-627 | ||
| 005 | 20240307044943.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231023s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cells12182315 |2 doi | |
| 035 | |a (DE-627)1867274558 | ||
| 035 | |a (DE-599)KXP1867274558 | ||
| 035 | |a (OCoLC)1425210116 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Sporkova, Alexandra |e VerfasserIn |0 (DE-588)1247776506 |0 (DE-627)1782386076 |4 aut | |
| 245 | 1 | 0 | |a Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cells |c Alexandra Sporkova, Taslima Nahar, Mingsi Cao, Subhajit Ghosh, Carla Sens-Albert, Prisca Amayi Patricia Friede, Anika Nagel, Jaafar Al-Hasani and Markus Hecker |
| 264 | 1 | |c 2023 | |
| 300 | |a 21 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Veröffentlicht: 19. September 2023 | ||
| 500 | |a Gesehen am 23.10.2023 | ||
| 520 | |a In arteries and arterioles, a chronic increase in blood pressure raises wall tension. This continuous biomechanical strain causes a change in gene expression in vascular smooth muscle cells (VSMCs) that may lead to pathological changes. Here we have characterised the functional properties of lipoma-preferred partner (LPP), a Lin11-Isl1-Mec3 (LIM)-domain protein, which is most closely related to the mechanotransducer zyxin but selectively expressed by smooth muscle cells, including VSMCs in adult mice. VSMCs isolated from the aorta of LPP knockout (LPP-KO) mice displayed a higher rate of proliferation than their wildtype (WT) counterparts, and when cultured as three-dimensional spheroids, they revealed a higher expression of the proliferation marker Ki 67 and showed greater invasion into a collagen gel. Accordingly, the gelatinase activity was increased in LPP-KO but not WT spheroids. The LPP-KO spheroids adhering to the collagen gel responded with decreased contraction to potassium chloride. The relaxation response to caffeine and norepinephrine was also smaller in the LPP-KO spheroids than in their WT counterparts. The overexpression of zyxin in LPP-KO VSMCs resulted in a reversal to a more quiescent differentiated phenotype. In native VSMCs, i.e., in isolated perfused segments of the mesenteric artery (MA), the contractile responses of LPP-KO segments to potassium chloride, phenylephrine or endothelin-1 did not vary from those in isolated perfused WT segments. In contrast, the myogenic response of LPP-KO MA segments was significantly attenuated while zyxin-deficient MA segments displayed a normal myogenic response. We propose that LPP, which we found to be expressed solely in the medial layer of different arteries from adult mice, may play an important role in controlling the quiescent contractile phenotype of VSMCs. | ||
| 650 | 4 | |a LPP | |
| 650 | 4 | |a mechanosensitive genes | |
| 650 | 4 | |a mechanotransduction | |
| 650 | 4 | |a vascular remodelling | |
| 650 | 4 | |a VSMC | |
| 700 | 1 | |a Nahar, Taslima |e VerfasserIn |4 aut | |
| 700 | 1 | |a Cao, Mingsi |d 1994- |e VerfasserIn |0 (DE-588)1306942896 |0 (DE-627)1867275015 |4 aut | |
| 700 | 1 | |a Ghosh, Subhajit |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sens-Albert, Carla |e VerfasserIn |4 aut | |
| 700 | 1 | |a Friede, Prisca Amayi Patricia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nagel, Anika |e VerfasserIn |4 aut | |
| 700 | 1 | |a Al-Hasani, Jaafar |e VerfasserIn |0 (DE-588)1247777472 |0 (DE-627)178238748X |4 aut | |
| 700 | 1 | |a Hecker, Markus |d 1960- |e VerfasserIn |0 (DE-588)1071693344 |0 (DE-627)826266037 |0 (DE-576)433260319 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cells |d Basel : MDPI, 2012 |g 12(2023), 18, Artikel-ID 2315, Seite 1-21 |h Online-Ressource |w (DE-627)718622081 |w (DE-600)2661518-6 |w (DE-576)365412112 |x 2073-4409 |7 nnas |a Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cells |
| 773 | 1 | 8 | |g volume:12 |g year:2023 |g number:18 |g elocationid:2315 |g pages:1-21 |g extent:21 |a Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cells |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cells12182315 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2073-4409/12/18/2315 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20231023 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 1071693344 |a Hecker, Markus |m 1071693344:Hecker, Markus |d 50000 |d 53200 |e 50000PH1071693344 |e 53200PH1071693344 |k 0/50000/ |k 1/50000/53200/ |p 9 |y j | ||
| 998 | |g 1247777472 |a Al-Hasani, Jaafar |m 1247777472:Al-Hasani, Jaafar |d 50000 |d 53200 |e 50000PA1247777472 |e 53200PA1247777472 |k 0/50000/ |k 1/50000/53200/ |p 8 | ||
| 998 | |g 1306942896 |a Cao, Mingsi |m 1306942896:Cao, Mingsi |d 50000 |d 53200 |e 50000PC1306942896 |e 53200PC1306942896 |k 0/50000/ |k 1/50000/53200/ |p 3 | ||
| 998 | |g 1247776506 |a Sporkova, Alexandra |m 1247776506:Sporkova, Alexandra |d 50000 |d 53200 |e 50000PS1247776506 |e 53200PS1247776506 |k 0/50000/ |k 1/50000/53200/ |p 1 |x j | ||
| 999 | |a KXP-PPN1867274558 |e 4395662315 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"recId":"1867274558","title":[{"title_sort":"Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cells","title":"Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cells"}],"note":["Veröffentlicht: 19. September 2023","Gesehen am 23.10.2023"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2023","dateIssuedDisp":"2023"}],"physDesc":[{"extent":"21 S."}],"name":{"displayForm":["Alexandra Sporkova, Taslima Nahar, Mingsi Cao, Subhajit Ghosh, Carla Sens-Albert, Prisca Amayi Patricia Friede, Anika Nagel, Jaafar Al-Hasani and Markus Hecker"]},"person":[{"display":"Sporkova, Alexandra","given":"Alexandra","family":"Sporkova","role":"aut","roleDisplay":"VerfasserIn"},{"given":"Taslima","family":"Nahar","roleDisplay":"VerfasserIn","role":"aut","display":"Nahar, Taslima"},{"role":"aut","roleDisplay":"VerfasserIn","given":"Mingsi","family":"Cao","display":"Cao, Mingsi"},{"family":"Ghosh","given":"Subhajit","role":"aut","roleDisplay":"VerfasserIn","display":"Ghosh, Subhajit"},{"display":"Sens-Albert, Carla","role":"aut","roleDisplay":"VerfasserIn","given":"Carla","family":"Sens-Albert"},{"display":"Friede, Prisca Amayi Patricia","given":"Prisca Amayi Patricia","family":"Friede","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Nagel, Anika","given":"Anika","family":"Nagel","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","role":"aut","given":"Jaafar","family":"Al-Hasani","display":"Al-Hasani, Jaafar"},{"given":"Markus","family":"Hecker","role":"aut","roleDisplay":"VerfasserIn","display":"Hecker, Markus"}],"relHost":[{"disp":"Characterisation of lipoma-preferred partner as a novel mechanotransducer in vascular smooth muscle cellsCells","physDesc":[{"extent":"Online-Ressource"}],"part":{"pages":"1-21","extent":"21","issue":"18","text":"12(2023), 18, Artikel-ID 2315, Seite 1-21","volume":"12","year":"2023"},"origin":[{"publisherPlace":"Basel","dateIssuedKey":"2012","dateIssuedDisp":"2012-","publisher":"MDPI"}],"note":["Gesehen am 09.05.12"],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"subtitle":"open access journal","title_sort":"Cells","title":"Cells"}],"pubHistory":["1.2012 -"],"language":["eng"],"recId":"718622081","id":{"eki":["718622081"],"zdb":["2661518-6"],"issn":["2073-4409"]}}],"id":{"doi":["10.3390/cells12182315"],"eki":["1867274558"]}} | ||
| SRT | |a SPORKOVAALCHARACTERI2023 | ||