Expulsion mechanism of the substrate-translocating subunit in ECF transporters
Energy-coupling factor (ECF)-type transporters mediate the uptake of micronutrients in many bacteria. They consist of a substrate-translocating subunit (S-component) and an ATP-hydrolysing motor (ECF module) Previous data indicate that the S-component topples within the membrane to alternately expos...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
25 July 2023
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| In: |
Nature Communications
Year: 2023, Jahrgang: 14, Pages: 1-14 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-023-40266-1 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-023-40266-1 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-023-40266-1 
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| Verfasserangaben: | Chancievan Thangaratnarajah, Mark Nijland, Luís Borges-Araújo, Aike Jeucken, Jan Rheinberger, Siewert J. Marrink, Paulo C.T. Souza, Cristina Paulino & Dirk J. Slotboom |
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| 245 | 1 | 0 | |a Expulsion mechanism of the substrate-translocating subunit in ECF transporters |c Chancievan Thangaratnarajah, Mark Nijland, Luís Borges-Araújo, Aike Jeucken, Jan Rheinberger, Siewert J. Marrink, Paulo C.T. Souza, Cristina Paulino & Dirk J. Slotboom |
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| 520 | |a Energy-coupling factor (ECF)-type transporters mediate the uptake of micronutrients in many bacteria. They consist of a substrate-translocating subunit (S-component) and an ATP-hydrolysing motor (ECF module) Previous data indicate that the S-component topples within the membrane to alternately expose the binding site to either side of the membrane. In many ECF transporters, the substrate-free S-component can be expelled from the ECF module. Here we study this enigmatic expulsion step by cryogenic electron microscopy and reveal that ATP induces a concave-to-convex shape change of two long helices in the motor, thereby destroying the S-component’s docking site and allowing for its dissociation. We show that adaptation of the membrane morphology to the conformational state of the motor may favour expulsion of the substrate-free S-component when ATP is bound and docking of the substrate-loaded S-component after hydrolysis. Our work provides a picture of bilayer-assisted chemo-mechanical coupling in the transport cycle of ECF transporters. | ||
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