Endosomal egress and intercellular transmission of hepatic ApoE-containing lipoproteins and its exploitation by the hepatitis C virus
Liver-generated plasma Apolipoprotein E (ApoE)-containing lipoproteins (LPs) (ApoE-LPs) play central roles in lipid transport and metabolism. Perturbations of ApoE can result in several metabolic disorders and ApoE genotypes have been associated with multiple diseases. ApoE is synthesized at the end...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 28, 2023
|
| Ausgabe: | Version 2 |
| In: |
PLoS pathogens
Year: 2023, Jahrgang: 19, Heft: 7, Pages: 1-40 |
| ISSN: | 1553-7374 |
| DOI: | 10.1371/journal.ppat.1011052 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1371/journal.ppat.1011052 Verlag, kostenfrei, Volltext: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011052 
|
| Verfasserangaben: | Minh-Tu Pham, Ji-Young Lee, Christian Ritter, Roman Thielemann, Janis Meyer, Uta Haselmann, Charlotta Funaya, Vibor Laketa, Karl Rohr, Ralf Bartenschlager |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1870252233 | ||
| 003 | DE-627 | ||
| 005 | 20240307042123.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231114s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.ppat.1011052 |2 doi | |
| 035 | |a (DE-627)1870252233 | ||
| 035 | |a (DE-599)KXP1870252233 | ||
| 035 | |a (OCoLC)1425209661 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Pham, Minh Tu |d 1989- |e VerfasserIn |0 (DE-588)1252772262 |0 (DE-627)1794415564 |4 aut | |
| 245 | 1 | 0 | |a Endosomal egress and intercellular transmission of hepatic ApoE-containing lipoproteins and its exploitation by the hepatitis C virus |c Minh-Tu Pham, Ji-Young Lee, Christian Ritter, Roman Thielemann, Janis Meyer, Uta Haselmann, Charlotta Funaya, Vibor Laketa, Karl Rohr, Ralf Bartenschlager |
| 250 | |a Version 2 | ||
| 264 | 1 | |c July 28, 2023 | |
| 300 | |a 40 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.11.2023 | ||
| 520 | |a Liver-generated plasma Apolipoprotein E (ApoE)-containing lipoproteins (LPs) (ApoE-LPs) play central roles in lipid transport and metabolism. Perturbations of ApoE can result in several metabolic disorders and ApoE genotypes have been associated with multiple diseases. ApoE is synthesized at the endoplasmic reticulum and transported to the Golgi apparatus for LP assembly; however, the ApoE-LPs transport pathway from there to the plasma membrane is largely unknown. Here, we established an integrative imaging approach based on a fully functional fluorescently tagged ApoE. We found that newly synthesized ApoE-LPs accumulate in CD63-positive endosomes of hepatocytes. In addition, we observed the co-egress of ApoE-LPs and CD63-positive intraluminal vesicles (ILVs), which are precursors of extracellular vesicles (EVs), along the late endosomal trafficking route in a microtubule-dependent manner. A fraction of ApoE-LPs associated with CD63-positive EVs appears to be co-transmitted from cell to cell. Given the important role of ApoE in viral infections, we employed as well-studied model the hepatitis C virus (HCV) and found that the viral replicase component nonstructural protein 5A (NS5A) is enriched in ApoE-containing ILVs. Interaction between NS5A and ApoE is required for the efficient release of ILVs containing HCV RNA. These vesicles are transported along the endosomal ApoE egress pathway. Taken together, our data argue for endosomal egress and transmission of hepatic ApoE-LPs, a pathway that is hijacked by HCV. Given the more general role of EV-mediated cell-to-cell communication, these insights provide new starting points for research into the pathophysiology of ApoE-related metabolic and infection-related disorders. | ||
| 650 | 4 | |a Confocal microscopy | |
| 650 | 4 | |a Endosomes | |
| 650 | 4 | |a Hepatocytes | |
| 650 | 4 | |a Lipids | |
| 650 | 4 | |a RNA structure | |
| 650 | 4 | |a Secretion | |
| 650 | 4 | |a Vesicles | |
| 650 | 4 | |a Viral replication | |
| 700 | 1 | |a Lee, Ji Young |e VerfasserIn |0 (DE-588)1058274570 |0 (DE-627)79668037X |0 (DE-576)414323610 |4 aut | |
| 700 | 1 | |a Ritter, Christian |d 1989- |e VerfasserIn |0 (DE-588)1191138550 |0 (DE-627)1669590356 |4 aut | |
| 700 | 1 | |a Thielemann, Roman |e VerfasserIn |0 (DE-588)1309865337 |0 (DE-627)1870264029 |4 aut | |
| 700 | 1 | |a Meyer, Janis |d 1994- |e VerfasserIn |0 (DE-588)1309865612 |0 (DE-627)187026441X |4 aut | |
| 700 | 1 | |a Haselmann, Uta |e VerfasserIn |0 (DE-588)1163130931 |0 (DE-627)1027359515 |0 (DE-576)507811127 |4 aut | |
| 700 | 1 | |a Funaya, Charlotta |e VerfasserIn |0 (DE-588)1181896231 |0 (DE-627)1662437099 |4 aut | |
| 700 | 1 | |a Laketa, Vibor |e VerfasserIn |0 (DE-588)132778068 |0 (DE-627)526931523 |0 (DE-576)299406830 |4 aut | |
| 700 | 1 | |a Rohr, Karl |e VerfasserIn |0 (DE-588)137474466 |0 (DE-627)695829440 |0 (DE-576)303788593 |4 aut | |
| 700 | 1 | |a Bartenschlager, Ralf |d 1958- |e VerfasserIn |0 (DE-588)1058097989 |0 (DE-627)796390509 |0 (DE-576)168706067 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a Public Library of Science |t PLoS pathogens |d Lawrence, Kan. : PLoS, 2005 |g 19(2023), 7, Artikel-ID e1011052, Seite 1-40 |h Online-Ressource |w (DE-627)501074422 |w (DE-600)2205412-1 |w (DE-576)273890530 |x 1553-7374 |7 nnas |
| 773 | 1 | 8 | |g volume:19 |g year:2023 |g number:7 |g elocationid:e1011052 |g pages:1-40 |g extent:40 |a Endosomal egress and intercellular transmission of hepatic ApoE-containing lipoproteins and its exploitation by the hepatitis C virus |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.ppat.1011052 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011052 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20231114 | ||
| 993 | |a Article | ||
| 994 | |a 2023 | ||
| 998 | |g 1058097989 |a Bartenschlager, Ralf |m 1058097989:Bartenschlager, Ralf |d 910000 |d 911700 |e 910000PB1058097989 |e 911700PB1058097989 |k 0/910000/ |k 1/910000/911700/ |p 10 |y j | ||
| 998 | |g 137474466 |a Rohr, Karl |m 137474466:Rohr, Karl |d 160000 |d 160100 |e 160000PR137474466 |e 160100PR137474466 |k 0/160000/ |k 1/160000/160100/ |p 9 | ||
| 998 | |g 132778068 |a Laketa, Vibor |m 132778068:Laketa, Vibor |d 910000 |d 911700 |e 910000PL132778068 |e 911700PL132778068 |k 0/910000/ |k 1/910000/911700/ |p 8 | ||
| 998 | |g 1181896231 |a Funaya, Charlotta |m 1181896231:Funaya, Charlotta |p 7 | ||
| 998 | |g 1163130931 |a Haselmann, Uta |m 1163130931:Haselmann, Uta |p 6 | ||
| 998 | |g 1309865612 |a Meyer, Janis |m 1309865612:Meyer, Janis |d 910000 |d 911700 |e 910000PM1309865612 |e 911700PM1309865612 |k 0/910000/ |k 1/910000/911700/ |p 5 | ||
| 998 | |g 1309865337 |a Thielemann, Roman |m 1309865337:Thielemann, Roman |p 4 | ||
| 998 | |g 1191138550 |a Ritter, Christian |m 1191138550:Ritter, Christian |d 160000 |e 160000PR1191138550 |k 0/160000/ |p 3 | ||
| 998 | |g 1058274570 |a Lee, Ji Young |m 1058274570:Lee, Ji Young |p 2 | ||
| 998 | |g 1252772262 |a Pham, Minh Tu |m 1252772262:Pham, Minh Tu |d 910000 |d 911700 |e 910000PP1252772262 |e 911700PP1252772262 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j | ||
| 999 | |a KXP-PPN1870252233 |e 4406601589 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"origin":[{"publisherPlace":"Lawrence, Kan.","publisher":"PLoS","dateIssuedKey":"2005","dateIssuedDisp":"2005-"}],"titleAlt":[{"title":"Pathogens"}],"id":{"zdb":["2205412-1"],"issn":["1553-7374"],"eki":["501074422"]},"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 20.11.2020"],"part":{"extent":"40","volume":"19","issue":"7","year":"2023","pages":"1-40","text":"19(2023), 7, Artikel-ID e1011052, Seite 1-40"},"name":{"displayForm":["Public Library of Science"]},"corporate":[{"roleDisplay":"VerfasserIn","display":"Public Library of Science","role":"aut"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"pubHistory":["1.2005,Sept. -"],"recId":"501074422","disp":"Public Library of SciencePLoS pathogens","title":[{"title_sort":"PLoS pathogens","title":"PLoS pathogens"}]}],"id":{"eki":["1870252233"],"doi":["10.1371/journal.ppat.1011052"]},"origin":[{"dateIssuedDisp":"July 28, 2023","edition":"Version 2","dateIssuedKey":"2023"}],"person":[{"roleDisplay":"VerfasserIn","family":"Pham","display":"Pham, Minh Tu","role":"aut","given":"Minh Tu"},{"display":"Lee, Ji Young","role":"aut","given":"Ji Young","roleDisplay":"VerfasserIn","family":"Lee"},{"given":"Christian","role":"aut","display":"Ritter, Christian","family":"Ritter","roleDisplay":"VerfasserIn"},{"family":"Thielemann","roleDisplay":"VerfasserIn","display":"Thielemann, Roman","role":"aut","given":"Roman"},{"roleDisplay":"VerfasserIn","family":"Meyer","display":"Meyer, Janis","role":"aut","given":"Janis"},{"display":"Haselmann, Uta","role":"aut","given":"Uta","family":"Haselmann","roleDisplay":"VerfasserIn"},{"role":"aut","display":"Funaya, Charlotta","given":"Charlotta","roleDisplay":"VerfasserIn","family":"Funaya"},{"given":"Vibor","role":"aut","display":"Laketa, Vibor","family":"Laketa","roleDisplay":"VerfasserIn"},{"given":"Karl","display":"Rohr, Karl","role":"aut","roleDisplay":"VerfasserIn","family":"Rohr"},{"roleDisplay":"VerfasserIn","family":"Bartenschlager","given":"Ralf","display":"Bartenschlager, Ralf","role":"aut"}],"note":["Gesehen am 14.11.2023"],"physDesc":[{"extent":"40 S."}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Minh-Tu Pham, Ji-Young Lee, Christian Ritter, Roman Thielemann, Janis Meyer, Uta Haselmann, Charlotta Funaya, Vibor Laketa, Karl Rohr, Ralf Bartenschlager"]},"title":[{"title_sort":"Endosomal egress and intercellular transmission of hepatic ApoE-containing lipoproteins and its exploitation by the hepatitis C virus","title":"Endosomal egress and intercellular transmission of hepatic ApoE-containing lipoproteins and its exploitation by the hepatitis C virus"}],"recId":"1870252233"} | ||
| SRT | |a PHAMMINHTUENDOSOMALE2820 | ||