Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril
The aggregation of antibody light chains is linked to systemic light chain (AL) amyloidosis, a disease where amyloid deposits frequently affect the heart and the kidney. We here investigate fibrils from the λ-III FOR005 light chain (LC), which is derived from an AL-patient with severe cardiac involv...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2021
|
| In: |
Biomolecular NMR assignments
Year: 2021, Jahrgang: 15, Heft: 1, Pages: 9-16 |
| ISSN: | 1874-270X |
| DOI: | 10.1007/s12104-020-09975-2 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1007/s12104-020-09975-2
|
| Verfasserangaben: | Tejaswini Pradhan, Karthikeyan Annamalai, Riddhiman Sarkar, Ute Hegenbart, Stefan Schönland, Marcus Fändrich, Bernd Reif |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1870862961 | ||
| 003 | DE-627 | ||
| 005 | 20250115022604.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231122s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12104-020-09975-2 |2 doi | |
| 035 | |a (DE-627)1870862961 | ||
| 035 | |a (DE-599)KXP1870862961 | ||
| 035 | |a (OCoLC)1425217590 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Pradhan, Tejaswini |e VerfasserIn |0 (DE-588)1226311784 |0 (DE-627)1747381667 |4 aut | |
| 245 | 1 | 0 | |a Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril |c Tejaswini Pradhan, Karthikeyan Annamalai, Riddhiman Sarkar, Ute Hegenbart, Stefan Schönland, Marcus Fändrich, Bernd Reif |
| 246 | 3 | 0 | |a lambda III λ |
| 246 | 3 | 3 | |a Solid state NMR assignments of a human lambda-III immunoglobulin light chain amyloid fibril |
| 264 | 1 | |c 2021 | |
| 300 | |b Illustrationen | ||
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Online veröffentlicht: 18. September 2020 | ||
| 500 | |a Gesehen am 22.11.2023 | ||
| 520 | |a The aggregation of antibody light chains is linked to systemic light chain (AL) amyloidosis, a disease where amyloid deposits frequently affect the heart and the kidney. We here investigate fibrils from the λ-III FOR005 light chain (LC), which is derived from an AL-patient with severe cardiac involvement. In FOR005, five residues are mutated with respect to its closest germline gene segment IGLV3-19 and IGLJ3. All mutations are located close to the complementarity determining regions (CDRs). The sequence segments responsible for the fibril formation are not yet known. We use fibrils extracted from the heart of this particular amyloidosis patient as seeds to prepare fibrils for solid-state NMR. We show that the seeds induce the formation of a specific fibril structure from the biochemically produced protein. We have assigned the fibril core region of the FOR005-derived fibrils and characterized the secondary structure propensity of the observed amino acids. As the primary structure of the aggregated patient protein is different for every AL patient, it is important to study, analyze and report a greater number of light chain sequences associated with AL amyloidosis. | ||
| 650 | 4 | |a AL amyloidosis | |
| 650 | 4 | |a Solid state NMR | |
| 650 | 4 | |a Variable light chain fibrils | |
| 700 | 1 | |a Annamalai, Karthikeyan |e VerfasserIn |4 aut | |
| 700 | 1 | |8 1\p |a Sarkar, Riddhiman |e VerfasserIn |0 (DE-588)1275936741 |0 (DE-627)1900628627 |4 aut | |
| 700 | 1 | |a Hegenbart, Ute |e VerfasserIn |0 (DE-588)1028373708 |0 (DE-627)73062157X |0 (DE-576)35901903X |4 aut | |
| 700 | 1 | |a Schönland, Stefan |d 1969- |e VerfasserIn |0 (DE-588)122405226 |0 (DE-627)705896137 |0 (DE-576)293255792 |4 aut | |
| 700 | 1 | |a Fändrich, Marcus |e VerfasserIn |4 aut | |
| 700 | 1 | |8 2\p |a Reif, Bernd |d 1968- |e VerfasserIn |0 (DE-588)12039054X |0 (DE-627)696628082 |0 (DE-576)292193394 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomolecular NMR assignments |d Dordrecht [u.a.] : Springer Netherlands, 2007 |g 15(2021), 1, Seite 9-16 |h Online-Ressource |w (DE-627)545787599 |w (DE-600)2388861-1 |w (DE-576)284927104 |x 1874-270X |7 nnas |a Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril |
| 773 | 1 | 8 | |g volume:15 |g year:2021 |g number:1 |g pages:9-16 |g extent:8 |a Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s12104-020-09975-2 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 2\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20231122 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 122405226 |a Schönland, Stefan |m 122405226:Schönland, Stefan |d 910000 |d 910100 |d 50000 |e 910000PS122405226 |e 910100PS122405226 |e 50000PS122405226 |k 0/910000/ |k 1/910000/910100/ |k 0/50000/ |p 5 | ||
| 998 | |g 1028373708 |a Hegenbart, Ute |m 1028373708:Hegenbart, Ute |d 910000 |d 910100 |d 50000 |e 910000PH1028373708 |e 910100PH1028373708 |e 50000PH1028373708 |k 0/910000/ |k 1/910000/910100/ |k 0/50000/ |p 4 | ||
| 999 | |a KXP-PPN1870862961 |e 4415590004 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1007/s12104-020-09975-2"],"eki":["1870862961"]},"physDesc":[{"noteIll":"Illustrationen","extent":"8 S."}],"name":{"displayForm":["Tejaswini Pradhan, Karthikeyan Annamalai, Riddhiman Sarkar, Ute Hegenbart, Stefan Schönland, Marcus Fändrich, Bernd Reif"]},"relHost":[{"title":[{"title_sort":"Biomolecular NMR assignments","title":"Biomolecular NMR assignments"}],"note":["Gesehen am 16.11.13"],"origin":[{"publisherPlace":"Dordrecht [u.a.]","dateIssuedDisp":"2007-","publisher":"Springer Netherlands","dateIssuedKey":"2007"}],"pubHistory":["1.2007 -"],"disp":"Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibrilBiomolecular NMR assignments","type":{"media":"Online-Ressource","bibl":"periodical"},"physDesc":[{"extent":"Online-Ressource"}],"recId":"545787599","id":{"eki":["545787599"],"issn":["1874-270X"],"zdb":["2388861-1"]},"language":["eng"],"part":{"volume":"15","extent":"8","year":"2021","pages":"9-16","issue":"1","text":"15(2021), 1, Seite 9-16"}}],"recId":"1870862961","type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril","title":"Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril"}],"note":["Online veröffentlicht: 18. September 2020","Gesehen am 22.11.2023"],"origin":[{"dateIssuedDisp":"2021","dateIssuedKey":"2021"}],"titleAlt":[{"title":"Solid state NMR assignments of a human lambda-III immunoglobulin light chain amyloid fibril"}],"language":["eng"],"person":[{"role":"aut","given":"Tejaswini","display":"Pradhan, Tejaswini","family":"Pradhan"},{"given":"Karthikeyan","role":"aut","display":"Annamalai, Karthikeyan","family":"Annamalai"},{"family":"Sarkar","display":"Sarkar, Riddhiman","role":"aut","given":"Riddhiman"},{"family":"Hegenbart","display":"Hegenbart, Ute","given":"Ute","role":"aut"},{"role":"aut","given":"Stefan","family":"Schönland","display":"Schönland, Stefan"},{"family":"Fändrich","display":"Fändrich, Marcus","role":"aut","given":"Marcus"},{"given":"Bernd","role":"aut","display":"Reif, Bernd","family":"Reif"}]} | ||
| SRT | |a PRADHANTEJSOLIDSTATE2021 | ||