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Cholesterol in the viral membrane is a molecular switch governing HIV-1 Env clustering

HIV-1 entry requires the redistribution of envelope glycoproteins (Env) into a cluster and the presence of cholesterol (chol) in the viral membrane. However, the molecular mechanisms underlying the specific role of chol in infectivity and the driving force behind Env clustering remain unknown. Here,...

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Main Authors: Nieto-Garai, Jon Ander (Author) , Arboleya, Aroa (Author) , Otaegi, Sara (Author) , Chojnacki, Jakub (Author) , Casas, Josefina (Author) , Fabriàs, Gemma (Author) , Contreras, F-Xabier (Author) , Kräusslich, Hans-Georg (Author) , Lorizate, Maier (Author)
Format: Article (Journal)
Language:English
Published: 2021
In: Advanced science
Year: 2021, Volume: 8, Issue: 3, Pages: 1-12
ISSN:2198-3844
DOI:10.1002/advs.202003468
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1002/advs.202003468
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202003468
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Author Notes:Jon Ander Nieto-Garai, Aroa Arboleya, Sara Otaegi, Jakub Chojnacki, Josefina Casas, Gemma Fabriàs, F-Xabier Contreras, Hans-Georg Kräusslich, and Maier Lorizate
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Summary:HIV-1 entry requires the redistribution of envelope glycoproteins (Env) into a cluster and the presence of cholesterol (chol) in the viral membrane. However, the molecular mechanisms underlying the specific role of chol in infectivity and the driving force behind Env clustering remain unknown. Here, gp41 is demonstrated to directly interact with chol in the viral membrane via residues 751-854 in the cytoplasmic tail (CT751-854). Super-resolution stimulated emission depletion (STED) nanoscopy analysis of Env distribution further demonstrates that both truncation of gp41 CT751-854 and depletion of chol leads to dispersion of Env clusters in the viral membrane and inhibition of virus entry. This work reveals a direct interaction of gp41 CT with chol and indicates that this interaction is an important orchestrator of Env clustering.
Item Description:First published: 21 December 2020
Gesehen am 23.11.2023
Physical Description:Online Resource
ISSN:2198-3844
DOI:10.1002/advs.202003468

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