Generation of patient-derived pediatric pilocytic astrocytoma in-vitro models using SV40 large T: evaluation of a modeling workflow
Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-gra...
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| Hauptverfasser: | , , , , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
24 November 2023
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| In: |
Journal of neuro-oncology
Year: 2023, Jahrgang: 165, Heft: 3, Pages: 467-478 |
| ISSN: | 1573-7373 |
| DOI: | 10.1007/s11060-023-04500-6 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11060-023-04500-6
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| Verfasserangaben: | Florian Selt, Ahmed El Damaty, Martin U. Schuhmann, Romain Sigaud, Jonas Ecker, Philipp Sievers, Daniela Kocher, Christel Herold-Mende, Ina Oehme, Andreas von Deimling, Stefan M. Pfister, Felix Sahm, David T.W. Jones, Olaf Witt, Till Milde |
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| 520 | |a Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-grade tumors fail in PA due to oncogene-induced senescence (OIS) driving tumor cells into arrest. Here, we describe a PA modeling workflow using the Simian Virus large T antigen (SV40-TAg) to circumvent OIS. | ||
| 650 | 4 | |a Circumvention of OIS | |
| 650 | 4 | |a In-vitro models | |
| 650 | 4 | |a Inducible SV40 large T | |
| 650 | 4 | |a Pediatric low-grade glioma cell lines | |
| 650 | 4 | |a Pilocytic astrocytoma | |
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| 773 | 0 | 8 | |i Enthalten in |t Journal of neuro-oncology |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1983 |g 165(2023), 3 vom: Nov., Seite 467-478 |h Online-Ressource |w (DE-627)32046122X |w (DE-600)2007293-4 |w (DE-576)107058944 |x 1573-7373 |7 nnas |a Generation of patient-derived pediatric pilocytic astrocytoma in-vitro models using SV40 large T evaluation of a modeling workflow |
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