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The Efg1-Bud22 dimer associates with the U14 snoRNP contacting the 5′ rRNA domain of an early 90S pre-ribosomal particle

The DEAD-box helicase Dbp4 plays an essential role during the early assembly of the 40S ribosome, which is only poorly understood to date. By applying the yeast two-hybrid method and biochemical approaches, we discovered that Dbp4 interacts with the Efg1-Bud22 dimer. Both factors associate with earl...

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Bibliographic Details
Main Authors: Beine-Golovchuk, Olga (Author) , Kallas, Martina (Author) , Kunze, Ruth (Author) , Griesel, Sabine (Author) , Baßler, Jochen (Author)
Format: Article (Journal)
Language:English
Published: January 2024
In: Nucleic acids research
Year: 2024, Volume: 52, Issue: 21, Pages: 431-447
ISSN:1362-4962
DOI:10.1093/nar/gkad1109
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1093/nar/gkad1109
Verlag, kostenfrei, Volltext: https://www.webofscience.com/api/gateway?GWVersion=2&SrcAuth=DynamicDOIArticle&SrcApp=WOS&KeyAID=10.1093%2Fnar%2Fgkad1109&DestApp=DOI&SrcAppSID=EUW1ED0B7AExZqY25qS2oQikhceCR&SrcJTitle=NUCLEIC+ACIDS+RESEARCH&DestDOIRegistrantName=Oxford+University+Press
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Author Notes:Olga Beine-Golovchuk, Martina Kallas, Ruth Kunze, Sabine Griesel and Jochen Baßler
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Summary:The DEAD-box helicase Dbp4 plays an essential role during the early assembly of the 40S ribosome, which is only poorly understood to date. By applying the yeast two-hybrid method and biochemical approaches, we discovered that Dbp4 interacts with the Efg1-Bud22 dimer. Both factors associate with early pre-90S particles and smaller complexes, each characterized by a high presence of the U14 snoRNA. A crosslink analysis of Bud22 revealed its contact to the U14 snoRNA and the 5 ' domain of the nascent 18S rRNA, close to its U14 snoRNA hybridization site. Moreover, depletion of Bud22 or Efg1 specifically affects U14 snoRNA association with pre-ribosomal complexes. Accordingly, we concluded that the role of the Efg1-Bud22 dimer is linked to the U14 snoRNA function on early 90S ribosome intermediates chaperoning the 5 ' domain of the nascent 18S rRNA. The successful rRNA folding of the 5 ' domain and the release of Efg1, Bud22, Dpb4, U14 snoRNA and associated snoRNP factors allows the subsequent recruitment of the Kre33-Bfr2-Enp2-Lcp5 module towards the 90S pre-ribosome. Graphical Abstract
Item Description:Gesehen am 23.01.2024
Online vorab veröffentlicht: 24. November 2023
Physical Description:Online Resource
ISSN:1362-4962
DOI:10.1093/nar/gkad1109

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