The Efg1-Bud22 dimer associates with the U14 snoRNP contacting the 5′ rRNA domain of an early 90S pre-ribosomal particle
The DEAD-box helicase Dbp4 plays an essential role during the early assembly of the 40S ribosome, which is only poorly understood to date. By applying the yeast two-hybrid method and biochemical approaches, we discovered that Dbp4 interacts with the Efg1-Bud22 dimer. Both factors associate with earl...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
January 2024
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| In: |
Nucleic acids research
Year: 2024, Jahrgang: 52, Heft: 21, Pages: 431-447 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkad1109 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1093/nar/gkad1109 Verlag, kostenfrei, Volltext: https://www.webofscience.com/api/gateway?GWVersion=2&SrcAuth=DynamicDOIArticle&SrcApp=WOS&KeyAID=10.1093%2Fnar%2Fgkad1109&DestApp=DOI&SrcAppSID=EUW1ED0B7AExZqY25qS2oQikhceCR&SrcJTitle=NUCLEIC+ACIDS+RESEARCH&DestDOIRegistrantName=Oxford+University+Press 
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| Verfasserangaben: | Olga Beine-Golovchuk, Martina Kallas, Ruth Kunze, Sabine Griesel and Jochen Baßler |
MARC
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| 520 | |a The DEAD-box helicase Dbp4 plays an essential role during the early assembly of the 40S ribosome, which is only poorly understood to date. By applying the yeast two-hybrid method and biochemical approaches, we discovered that Dbp4 interacts with the Efg1-Bud22 dimer. Both factors associate with early pre-90S particles and smaller complexes, each characterized by a high presence of the U14 snoRNA. A crosslink analysis of Bud22 revealed its contact to the U14 snoRNA and the 5 ' domain of the nascent 18S rRNA, close to its U14 snoRNA hybridization site. Moreover, depletion of Bud22 or Efg1 specifically affects U14 snoRNA association with pre-ribosomal complexes. Accordingly, we concluded that the role of the Efg1-Bud22 dimer is linked to the U14 snoRNA function on early 90S ribosome intermediates chaperoning the 5 ' domain of the nascent 18S rRNA. The successful rRNA folding of the 5 ' domain and the release of Efg1, Bud22, Dpb4, U14 snoRNA and associated snoRNP factors allows the subsequent recruitment of the Kre33-Bfr2-Enp2-Lcp5 module towards the 90S pre-ribosome. Graphical Abstract | ||
| 650 | 4 | |a BIOGENESIS | |
| 650 | 4 | |a HELICASE DBP4 | |
| 650 | 4 | |a IDENTIFICATION | |
| 650 | 4 | |a PRERIBOSOME | |
| 650 | 4 | |a PROCESSOME | |
| 650 | 4 | |a PROTEINS | |
| 650 | 4 | |a SMALL NUCLEOLAR RNA | |
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| 650 | 4 | |a U3 SNORNA | |
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