Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels [data]
This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabric...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Datenbank Forschungsdaten |
| Sprache: | Englisch |
| Veröffentlicht: |
Heidelberg
Universität
2024-02-16
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| DOI: | 10.11588/data/V2XXS8 |
| Schlagworte: | |
| Online-Zugang: | Resolving-System, kostenfrei, Volltext: https://doi.org/10.11588/data/V2XXS8 Verlag, kostenfrei, Volltext: https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/data/V2XXS8 
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| Verfasserangaben: | Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, Eva Blasco |
MARC
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| 245 | 1 | 0 | |a Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels [data] |c Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, Eva Blasco |
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| 500 | |a Finanziert durch: Spotlight Project “Synthetic Immunology” of the Flagship Initiative Engineering Molecular Systems (FI EMS; funded by the BMBF and the Ministry of Science Baden-Württemberg); Excellence Cluster “3D Matter Made to Order” (funded by the DFG): EXC 2082/1 390761711; ERC Consolidator Grant PHOTOMECH: Grant no. 101001797; Volkswagen Foundation Initiative “Life?,”: Az. 96733 | ||
| 500 | |a Gesehen am 21.02.2024 | ||
| 520 | |a This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabrication. The printable cell-adhesive PEG-based material includes an RGD-containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of our system, we showcase the fabrication of cell-adhesive 2.5D and 3D structures, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments. | ||
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