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CRISPR-Fortschritte: Schnitt für Schnitt zu neuen Möglichkeiten

CRISPR/Cas9 systems and recently established base editors are essential tools for precise, targeted genome editing for translational and basic research applications. Here we present small, easily combined improvements to reach editing versatility and enhanced efficiency. This is achieved by improved...

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Hauptverfasser: Pakari, Kaisa (VerfasserIn) , Wittbrodt, Joachim (VerfasserIn) , Thumberger, Thomas (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Deutsch
Veröffentlicht: 21 February 2023
In: Biospektrum
Year: 2023, Jahrgang: 29, Heft: 1, Pages: 25-28
ISSN:1868-6249
DOI:10.1007/s12268-023-1893-z
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s12268-023-1893-z
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Verfasserangaben:Kaisa Pakari, Joachim Wittbrodt, Thomas Thumberger
Beschreibung
Zusammenfassung:CRISPR/Cas9 systems and recently established base editors are essential tools for precise, targeted genome editing for translational and basic research applications. Here we present small, easily combined improvements to reach editing versatility and enhanced efficiency. This is achieved by improved nuclear localization of Cas9, protected DNA sequences for homology directed repair and a combinatorial use of base editors to reach initially inaccessible target sites.
Beschreibung:Gesehen am 26.02.2024
Beschreibung:Online Resource
ISSN:1868-6249
DOI:10.1007/s12268-023-1893-z

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