The inceptionist’s guide to base editing: de novo PAM generation to reach initially inaccessible target-sites
Base editing by CRISPR crucially depends on the presence of a PAM in proper distance to the editing-site. Here we present and validate an efficient one-shot approach termed “inception”, relaxing these constraints. This is achieved by sequential, combinatorial base editing: de novo generated synonymo...
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| Main Authors: | , , |
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| Format: | Article (Journal) Chapter/Article |
| Language: | English |
| Published: |
July 07, 2022
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| In: |
bioRxiv beta
Year: 2022, Pages: 1-18 |
| DOI: | 10.1101/2022.07.07.499158 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1101/2022.07.07.499158 Verlag, kostenfrei, Volltext: https://www.biorxiv.org/content/10.1101/2022.07.07.499158v1 
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| Author Notes: | Kaisa Pakari, Joachim Wittbrodt, Thomas Thumberger |
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| 520 | |a Base editing by CRISPR crucially depends on the presence of a PAM in proper distance to the editing-site. Here we present and validate an efficient one-shot approach termed “inception”, relaxing these constraints. This is achieved by sequential, combinatorial base editing: de novo generated synonymous, non-synonymous or intronic PAM-sites facilitate subsequent base editing at nucleotide positions inaccessible before, increasing the targeting range of highly precise editing approaches. | ||
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