Cover Image

Circular RNAs

Show other versions (1)

Purification of circular RNAs using poly(A) tailing followed by RNase R digestion -- State of the art circular RNA analytics using the circtools software suite -- Exploring circular RNA profile and expression in extracellular vesicles -- Sanger sequencing to determine the full-length sequence of cir...

Full description

Saved in:
Bibliographic Details
Other Authors: Dieterich, Christoph (Editor) , Baudet, Marie-Laure (Editor)
Format: Book/Monograph
Language:English
Published: New York, NY Springer US 2024
New York, NY Imprint: Humana 2024
Edition:2nd ed. 2024
Series:Methods in Molecular Biology 2765
DOI:10.1007/978-1-0716-3678-7
Online Access:Resolving-System, lizenzpflichtig: https://doi.org/10.1007/978-1-0716-3678-7
Get full text
Author Notes:edited by Christoph Dieterich, Marie-Laure Baudet

MARC

LEADER 00000cam a2200000 c 4500
001 1881680924
003 DE-627
005 20260209085911.0
007 cr uuu---uuuuu
008 240227s2024 xxu|||||o 00| ||eng c
020 |a 9781071636787  |9 978-1-0716-3678-7 
024 7 |a 10.1007/978-1-0716-3678-7  |2 doi 
035 |a (DE-627)1881680924 
035 |a (DE-603)518691756 
035 |a (DE-599)KEP100849164 
035 |a (DE-He213)978-1-0716-3678-7 
035 |a (DE-627-1)100849164 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
044 |c XD-US 
072 7 |a PSAK  |2 bicssc 
072 7 |a SCI029000  |2 bisacsh 
082 0 |a 576.5  |2 23 
084 |a 32  |2 sdnb 
245 0 0 |a Circular RNAs  |c edited by Christoph Dieterich, Marie-Laure Baudet 
250 |a 2nd ed. 2024 
264 1 |a New York, NY  |b Springer US  |c 2024 
264 1 |a New York, NY  |b Imprint: Humana  |c 2024 
300 |a 1 Online-Ressource (XI, 326 p. 58 illus., 53 illus. in color.) 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
490 0 |a Methods in Molecular Biology  |v 2765 
520 |a Purification of circular RNAs using poly(A) tailing followed by RNase R digestion -- State of the art circular RNA analytics using the circtools software suite -- Exploring circular RNA profile and expression in extracellular vesicles -- Sanger sequencing to determine the full-length sequence of circular RNAs -- Droplet digital PCR for the detection and quantification of bona fide circRNAs -- Targeted sequencing of circular RNAs for Illumina-based counting and Nanopore structure determination -- Nanopore mediated sequencing of circular RNA -- In vivo tissue-specific knockdown of circRNAs using shRNAs in Drosophila Melanogaster -- Functional circular RNA screening via RfxCas13d/BSJ-gRNA system -- Base-editor-mediated circRNA knockout by targeting predominantly back-splice sites -- Directed Circularization of a Short RNA -- Engineering Synthetic circRNAs for Efficient CNS Expression -- Functional investigation of in vitro transcribed circular RNA and formulation into Lipid nanoparticles via microfluidic mixing -- Characterising post-transcriptional modifications of circRNAs to investigate biogenesis and translation -- Native circular RNA pulldown method to simultaneously profile RNA and protein interactions -- Screening and characterization of functional circRNAs in neuronal cultures. . 
520 |a This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA translation potential, and vaccines based on circular RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Circular RNAs, Second Edition aims to ensure successful results in the further study of this vital field. 
650 0 |a Genetics. 
700 1 |a Dieterich, Christoph  |d 1975-  |e HerausgeberIn  |0 (DE-588)130054844  |0 (DE-627)494359269  |0 (DE-576)297972448  |4 edt 
700 1 |a Baudet, Marie-Laure  |e HerausgeberIn  |0 (DE-588)1331095700  |0 (DE-627)1890255386  |4 edt 
776 1 |z 9781071636770 
776 1 |z 9781071636794 
776 1 |z 9781071636800 
776 0 8 |i Erscheint auch als  |n Druck-Ausgabe  |z 9781071636770 
776 0 8 |i Erscheint auch als  |n Druck-Ausgabe  |z 9781071636794 
776 0 8 |i Erscheint auch als  |n Druck-Ausgabe  |z 9781071636800 
776 0 8 |i Erscheint auch als  |n Druck-Ausgabe  |t Circular RNAs  |b Second editioan  |d New York : Humana Press, 2024  |h XI, 326 Seiten  |w (DE-627)1905620489  |z 9781071636770  |z 1071636774 
856 4 0 |u https://doi.org/10.1007/978-1-0716-3678-7  |m X:SPRINGER  |x Resolving-System  |z lizenzpflichtig  |7 1 
912 |a ZDB-2-SEB  |b 2024 
912 |a ZDB-2-PRO  |b 2024 
936 s n |a w100  |2 mzub 
951 |a BO 
992 |a 20260209 
993 |a Book 
994 |a 2024 
998 |g 130054844  |a Dieterich, Christoph  |m 130054844:Dieterich, Christoph  |d 910000  |d 910100  |e 910000PD130054844  |e 910100PD130054844  |k 0/910000/  |k 1/910000/910100/  |p 1  |x j 
999 |a KXP-PPN1881680924  |e 4911364904 
BIB |a Y 
JSO |a {"physDesc":[{"extent":"1 Online-Ressource (XI, 326 p. 58 illus., 53 illus. in color.)"}],"recId":"1881680924","origin":[{"publisher":"Springer US ; Imprint: Humana","edition":"2nd ed. 2024","publisherPlace":"New York, NY ; New York, NY","dateIssuedDisp":"2024","dateIssuedKey":"2024","editionNo":2}],"name":{"displayForm":["edited by Christoph Dieterich, Marie-Laure Baudet"]},"person":[{"display":"Dieterich, Christoph","family":"Dieterich","role":"edt","given":"Christoph"},{"display":"Baudet, Marie-Laure","family":"Baudet","given":"Marie-Laure","role":"edt"}],"type":{"bibl":"edited-book","media":"Online-Ressource"},"language":["eng"],"id":{"doi":["10.1007/978-1-0716-3678-7"],"isbn":["9781071636787"],"eki":["1881680924"]},"title":[{"title":"Circular RNAs","title_sort":"Circular RNAs"}]} 
SRT |a CIRCULARRN2024 

Similar Items