Identification of NAD-RNA species and ADPR-RNA decapping in Archaea
NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archa...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
21 November 2023
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| In: |
Nature Communications
Year: 2023, Jahrgang: 14, Pages: 1-12 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-023-43377-x |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-023-43377-x Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-023-43377-x 
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| Verfasserangaben: | José Vicente Gomes-Filho, Ruth Breuer, Hector Gabriel Morales-Filloy, Nadiia Pozhydaieva, Andreas Borst, Nicole Paczia, Jörg Soppa, Katharina Höfer, Andres Jäschke & Lennart Randau |
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| 245 | 1 | 0 | |a Identification of NAD-RNA species and ADPR-RNA decapping in Archaea |c José Vicente Gomes-Filho, Ruth Breuer, Hector Gabriel Morales-Filloy, Nadiia Pozhydaieva, Andreas Borst, Nicole Paczia, Jörg Soppa, Katharina Höfer, Andres Jäschke & Lennart Randau |
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| 520 | |a NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NAD-RNA decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPR-RNA decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in S. acidocaldarius total RNA. Deletion of the gene encoding the 5′−3′ exonuclease Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels. We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs, providing another layer of regulation for RNA turnover in archaeal cells. | ||
| 650 | 4 | |a Archaeal biology | |
| 650 | 4 | |a Archaeal physiology | |
| 650 | 4 | |a RNA modification | |
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