Characterizing medaka visual features using a high-throughput optomotor response assay
Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
March 31, 2024
|
| In: |
bioRxiv beta
Year: 2024, Pages: 1-26 |
| DOI: | 10.1101/2024.03.28.587314 |
| Online-Zugang: | Resolving-System, kostenfrei, Volltext: https://doi.org/10.1101/2024.03.28.587314 Verlag, kostenfrei, Volltext: https://www.biorxiv.org/content/10.1101/2024.03.28.587314v1 
|
| Verfasserangaben: | Risa Suzuki, Jia Zheng Woo, Thomas Thumberger, Gero Hofmann, Joachim Wittbrodt and Tinatini Tavhelidse-Suck |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1884690580 | ||
| 003 | DE-627 | ||
| 005 | 20240703161437.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240402s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2024.03.28.587314 |2 doi | |
| 035 | |a (DE-627)1884690580 | ||
| 035 | |a (DE-599)KXP1884690580 | ||
| 035 | |a (OCoLC)1443667831 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Suzuki, Risa |d 1994- |e VerfasserIn |0 (DE-588)1255234148 |0 (DE-627)1799332861 |4 aut | |
| 245 | 1 | 0 | |a Characterizing medaka visual features using a high-throughput optomotor response assay |c Risa Suzuki, Jia Zheng Woo, Thomas Thumberger, Gero Hofmann, Joachim Wittbrodt and Tinatini Tavhelidse-Suck |
| 264 | 1 | |c March 31, 2024 | |
| 300 | |b Illustrationen | ||
| 300 | |a 26 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.04.2024 | ||
| 520 | |a Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and the Fish Stripes software to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes. | ||
| 700 | 1 | |a Woo, Jia Zheng |e VerfasserIn |4 aut | |
| 700 | 1 | |a Thumberger, Thomas |e VerfasserIn |0 (DE-588)1026677068 |0 (DE-627)727045091 |0 (DE-576)354676075 |4 aut | |
| 700 | 1 | |a Hofmann, Gero |d 1973- |e VerfasserIn |0 (DE-588)1325124435 |0 (DE-627)1884798217 |4 aut | |
| 700 | 1 | |a Wittbrodt, Joachim |e VerfasserIn |0 (DE-588)1038250919 |0 (DE-627)756835356 |0 (DE-576)167753401 |4 aut | |
| 700 | 1 | |a Tavhelidse-Suck, Tinatini |e VerfasserIn |0 (DE-588)1124483764 |0 (DE-627)878199837 |0 (DE-576)482786531 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv beta |d Cold Spring Harbor : Cold Spring Harbor Laboratory, NY, 2013 |g (2024) vom: März, Artikel-ID 2024.03.28.587314, Seite 1-26 |h Online-Ressource |w (DE-627)783307497 |w (DE-600)2766415-6 |w (DE-576)404299903 |7 nnas |a Characterizing medaka visual features using a high-throughput optomotor response assay |
| 773 | 1 | 8 | |g year:2024 |g month:03 |g elocationid:2024.03.28.587314 |g pages:1-26 |g extent:26 |a Characterizing medaka visual features using a high-throughput optomotor response assay |
| 856 | 4 | 0 | |u https://doi.org/10.1101/2024.03.28.587314 |x Resolving-System |x Verlag |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.biorxiv.org/content/10.1101/2024.03.28.587314v1 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20240402 | ||
| 993 | |a Article | ||
| 994 | |a 2024 | ||
| 998 | |g 1124483764 |a Tavhelidse-Suck, Tinatini |m 1124483764:Tavhelidse-Suck, Tinatini |d 700000 |d 721000 |e 700000PT1124483764 |e 721000PT1124483764 |k 0/700000/ |k 1/700000/721000/ |p 6 |y j | ||
| 998 | |g 1038250919 |a Wittbrodt, Joachim |m 1038250919:Wittbrodt, Joachim |d 700000 |d 721000 |e 700000PW1038250919 |e 721000PW1038250919 |k 0/700000/ |k 1/700000/721000/ |p 5 | ||
| 998 | |g 1325124435 |a Hofmann, Gero |m 1325124435:Hofmann, Gero |d 700000 |d 721000 |e 700000PH1325124435 |e 721000PH1325124435 |k 0/700000/ |k 1/700000/721000/ |p 4 | ||
| 998 | |g 1026677068 |a Thumberger, Thomas |m 1026677068:Thumberger, Thomas |d 700000 |d 721000 |e 700000PT1026677068 |e 721000PT1026677068 |k 0/700000/ |k 1/700000/721000/ |p 3 | ||
| 998 | |g 1255234148 |a Suzuki, Risa |m 1255234148:Suzuki, Risa |d 700000 |d 721000 |e 700000PS1255234148 |e 721000PS1255234148 |k 0/700000/ |k 1/700000/721000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1884690580 |e 450627010X | ||
| BIB | |a Y | ||
| JSO | |a {"id":{"eki":["1884690580"],"doi":["10.1101/2024.03.28.587314"]},"name":{"displayForm":["Risa Suzuki, Jia Zheng Woo, Thomas Thumberger, Gero Hofmann, Joachim Wittbrodt and Tinatini Tavhelidse-Suck"]},"note":["Gesehen am 02.04.2024"],"type":{"bibl":"chapter","media":"Online-Ressource"},"language":["eng"],"title":[{"title":"Characterizing medaka visual features using a high-throughput optomotor response assay","title_sort":"Characterizing medaka visual features using a high-throughput optomotor response assay"}],"person":[{"role":"aut","given":"Risa","family":"Suzuki","display":"Suzuki, Risa"},{"family":"Woo","given":"Jia Zheng","role":"aut","display":"Woo, Jia Zheng"},{"given":"Thomas","role":"aut","family":"Thumberger","display":"Thumberger, Thomas"},{"display":"Hofmann, Gero","given":"Gero","role":"aut","family":"Hofmann"},{"display":"Wittbrodt, Joachim","role":"aut","given":"Joachim","family":"Wittbrodt"},{"display":"Tavhelidse-Suck, Tinatini","family":"Tavhelidse-Suck","given":"Tinatini","role":"aut"}],"relHost":[{"pubHistory":["2013 -"],"titleAlt":[{"title":"bio archive"}],"id":{"eki":["783307497"],"zdb":["2766415-6"]},"part":{"extent":"26","text":"(2024) vom: März, Artikel-ID 2024.03.28.587314, Seite 1-26","year":"2024","pages":"1-26"},"physDesc":[{"extent":"Online-Ressource"}],"recId":"783307497","disp":"Characterizing medaka visual features using a high-throughput optomotor response assaybioRxiv beta","origin":[{"publisherPlace":"Cold Spring Harbor","dateIssuedDisp":"2013-","dateIssuedKey":"2013","publisher":"Cold Spring Harbor Laboratory, NY"}],"language":["eng"],"type":{"bibl":"edited-book","media":"Online-Ressource"},"note":["Gesehen am 14.04.2014"],"title":[{"subtitle":"the preprint server for biology","title_sort":"bioRxiv beta","title":"bioRxiv beta"}]}],"origin":[{"dateIssuedKey":"2024","dateIssuedDisp":"March 31, 2024"}],"physDesc":[{"extent":"26 S.","noteIll":"Illustrationen"}],"recId":"1884690580"} | ||
| SRT | |a SUZUKIRISACHARACTERI3120 | ||