Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection
Using an ultra-sensitive fluorescence in situ hybridization (FISH) protocol, integration sites of the human immunodeficiency virus-1 (HIV-1) were mapped in two model cell lines for chronic HIV infection. In the promonocytic cell line U1/HIV-1, the two integrated copies of proviral HIV-DNA were locat...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1997
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| In: |
Journal of virological methods
Year: 1997, Volume: 65, Issue: 1, Pages: 19-25 |
| ISSN: | 1879-0984 |
| DOI: | 10.1016/S0166-0934(96)02164-7 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0166-0934(96)02164-7 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0166093496021647 
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| Author Notes: | Martin Deichmann, Martin Bentz, Rainer Haas |
| Summary: | Using an ultra-sensitive fluorescence in situ hybridization (FISH) protocol, integration sites of the human immunodeficiency virus-1 (HIV-1) were mapped in two model cell lines for chronic HIV infection. In the promonocytic cell line U1/HIV-1, the two integrated copies of proviral HIV-DNA were located on the short arm of one copy of chromosome X (band Xp21) and on a rearranged chromosome 6. In the T-cell line 8E5/LAV, the single copy of proviral HIV-DNA was integrated into chromosomal bands 13q14-q21, which are known to contain common fragile sites. In addition, ultra-sensitive FISH was used for the detection of HIV provirus sequences in interphase cells, achieving a high hybridization efficiency. This application allows the analysis of the HIV status on a single cell level and thus may become a useful tool for the study of HIV infection. |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 23. November 1999 Gesehen am 03.04.2024 |
| Physical Description: | Online Resource |
| ISSN: | 1879-0984 |
| DOI: | 10.1016/S0166-0934(96)02164-7 |