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Ultra-sensitive FISH is a useful tool for studying chronic HIV-1 infection

Using an ultra-sensitive fluorescence in situ hybridization (FISH) protocol, integration sites of the human immunodeficiency virus-1 (HIV-1) were mapped in two model cell lines for chronic HIV infection. In the promonocytic cell line U1/HIV-1, the two integrated copies of proviral HIV-DNA were locat...

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Hauptverfasser: Deichmann, Martin (VerfasserIn) , Bentz, Martin (VerfasserIn) , Haas, Rainer (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 1997
In: Journal of virological methods
Year: 1997, Jahrgang: 65, Heft: 1, Pages: 19-25
ISSN:1879-0984
DOI:10.1016/S0166-0934(96)02164-7
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0166-0934(96)02164-7
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0166093496021647
Volltext
Verfasserangaben:Martin Deichmann, Martin Bentz, Rainer Haas

MARC

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520 |a Using an ultra-sensitive fluorescence in situ hybridization (FISH) protocol, integration sites of the human immunodeficiency virus-1 (HIV-1) were mapped in two model cell lines for chronic HIV infection. In the promonocytic cell line U1/HIV-1, the two integrated copies of proviral HIV-DNA were located on the short arm of one copy of chromosome X (band Xp21) and on a rearranged chromosome 6. In the T-cell line 8E5/LAV, the single copy of proviral HIV-DNA was integrated into chromosomal bands 13q14-q21, which are known to contain common fragile sites. In addition, ultra-sensitive FISH was used for the detection of HIV provirus sequences in interphase cells, achieving a high hybridization efficiency. This application allows the analysis of the HIV status on a single cell level and thus may become a useful tool for the study of HIV infection. 
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