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MePMe-seq: antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing

Internal modifications of mRNA have emerged as widespread and versatile regulatory mechanism to control gene expression at the post-transcriptional level. Most of these modifications are methyl groups, making S-adenosyl-L-methionine (SAM) a central metabolic hub. Here we show that metabolic labeling...

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Main Authors: Hartstock, Katja (Author) , Kück, Nadine A. (Author) , Spacek, Petr (Author) , Ovcharenko, Anna (Author) , Huewel, Sabine (Author) , Cornelissen, Nicolas V. (Author) , Bollu, Amarnath (Author) , Dieterich, Christoph (Author) , Rentmeister, Andrea (Author)
Format: Article (Journal)
Language:English
Published: 07 November 2023
In: Nature Communications
Year: 2023, Volume: 14, Pages: 1-19
ISSN:2041-1723
DOI:10.1038/s41467-023-42832-z
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-023-42832-z
Verlag, lizenzpflichtig, Volltext: https://www.webofscience.com/api/gateway?GWVersion=2&SrcAuth=DOISource&SrcApp=WOS&KeyAID=10.1038%2Fs41467-023-42832-z&DestApp=DOI&SrcAppSID=EUW1ED0C77UzrgOaVNNCFjQlQ8fu9&SrcJTitle=NATURE+COMMUNICATIONS&DestDOIRegistrantName=Springer+Science+and+Business+Media+LLC
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Author Notes:Katja Hartstock, Nadine A. Kueck, Petr Spacek, Anna Ovcharenko, Sabine Huewel, Nicolas V. Cornelissen, Amarnath Bollu, Christoph Dieterich & Andrea Rentmeister

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